ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia
ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia
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DOI：
10.1056/nejmoa023143
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发表时间：
2003-05-01
影响因子：
158.5
通讯作者：
Montserrat, E
中科院分区：
文献类型：
--
作者：
Crespo, M;Bosch, F;Montserrat, E
背景：慢性淋巴细胞白血病（CLL）白血病细胞中免疫球蛋白重链可变区（IgV（subH））基因的突变状态是该疾病的重要预后因素。我们研究了ZAP-70的表达CLL细胞是否与IgV（亚H）突变状态，疾病进展，和survival.METHODS：ZAP-70的表达进行了分析，在T细胞和B细胞系和外周血样本从56例CLL与使用流式细胞术，蛋白质印迹法，免疫组化。结果：流式细胞术检测56例CLL患者中有32例患者的T细胞系细胞和白血病细胞中ZAP-70阳性。在所有至少20%的白血病细胞ZAP-70阳性的患者中，IgV（subH）未发生突变，而在24例不到20%的白血病细胞ZAP-70阳性的患者中，有21例发现IgV（subH）突变（P
BACKGROUND:The mutational status of immunoglobulin heavy-chain variable-region (IgV(sub H)) genes in the leukemic cells of chronic lymphocytic leukemia (CLL) is an important prognostic factor in the disease. We investigated whether the expression of ZAP-70 by CLL cells correlated with the IgV(sub H) mutational status, disease progression, and survival.METHODS:The expression of ZAP-70 was analyzed in T-cell and B-cell lines and in peripheral-blood samples from 56 patients with CLL with the use of flow cytometry, Western blotting, and immunohistochemistry. The results were correlated with the IgV(sub H) mutational status and clinical outcome.RESULTS:ZAP-70 was detected by flow-cytometric analysis in cells of T-cell lineage and in leukemic cells from 32 of 56 patients with CLL. In all patients in whom at least 20 percent of the leukemic cells were positive for ZAP-70, IgV(sub H) was unmutated, whereas IgV(sub H) mutations were found in 21 of 24 patients in whom less than 20 percent of the leukemic cells were positive for ZAP-70 (P