Fluorine-19 MRI for visualization and quantification of cell migration in a diabetes model
Fluorine-19 MRI for visualization and quantification of cell migration in a diabetes model
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DOI：
10.1002/mrm.21352
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发表时间：
2007-10-01
影响因子：
3.3
通讯作者：
Ahrens, Eric T.
中科院分区：
文献类型：
--
作者：
Srinivas, Mangala;Morel, Penelope A.;Ahrens, Eric T.
本文描述了一种用于可视化和定量特定细胞群的体内成像方法。用全氟聚醚纳米颗粒示踪剂在体外标记细胞，然后在细胞转移后使用F-19 MRI在体内检测细胞。19F MRI在没有背景的情况下选择性地只显示标记的细胞，而在同一成像过程中拍摄的传统H-1图像提供了解剖背景。使用非肥胖的糖尿病小鼠（已建立的1型糖尿病模型），获得的F-19 MRI数据显示了致糖尿病T细胞早期归巢行为。计算算法提供了胰腺中的T细胞计数。大约2%的移植细胞在48小时后回到胰腺。该技术允许对标记细胞进行明确的检测，并直接从体内图像进行定量。使用F-19光谱和荧光显微镜在切除的胰腺中验证了体内定量和细胞运输模式。标记过程不影响t细胞在体内的迁移。该成像平台适用于许多细胞类型和疾病模型，并有可能用于监测细胞疗法的贩运。
This article describes an in vivo imaging method for visualizing and quantifying a specific cell population. Cells are labeled ex vivo with a perfluoropolyether nanoparticle tracer agent and then detected in vivo using F-19 MRI following cell transfer. 19F MRI selectively visualizes only the labeled cells with no background, and a conventional H-1 image taken in the same imaging session provides anatomical context. Using the nonobese diabetic mouse, an established model of type 1 diabetes, F-19 MRI data were acquired showing the early homing behavior of diabetogenic T cells to the pancreas. A computational algorithm provided T cell counts in the pancreas. Approximately 2% of the transferred cells homed to the pancreas after 48 hr. The technique allows for both unambiguous detection of labeled cells and quantification directly from the in vivo images. The in vivo quantification and cell trafficking patterns were verified using F-19 spectroscopy and fluorescence microscopy in excised pancreata. The labeling procedure did not affect T-cell migration in vivo. This imaging platform is applicable to many cell types and disease models and can potentially be used for monitoring the trafficking of cellular therapeutics.