Spontaneous motor seizures of rats with kainate-induced epilepsy: effect of time of day and activity state
Spontaneous motor seizures of rats with kainate-induced epilepsy: effect of time of day and activity state
复制标题
DOI：
10.1016/s0920-1211(98)00127-2
复制
发表时间：
1999-05-01
影响因子：
2.2
通讯作者：
Dudek, FE
中科院分区：
文献类型：
--
作者：
Hellier, JL;Dudek, FE
红藻氨酸对大鼠的治疗可以导致类似于人类颞叶癫痫的慢性行为状态。我们测试了一种假设，即像一些患有癫痫的人类一样，红藻氨酸诱导的癫痫大鼠在不活动期间(即很少或没有意志性运动，包括明显的睡眠)比在活动期间(即明显的意志性运动，如行走、梳理、进食等)有更多的自发运动发作。大鼠腹腔注射(Ip)。每小时注射红藻氨酸(5 mg/kg)，诱发III/IV/V级癫痫发作大于或等于3h。视频监测癫痫发作行为(治疗后24小时5~6天，32只大鼠在3个月，23只大鼠在4个月)，观察癫痫发作的发生与光照与黑暗(12~12小时光暗周期)的关系以及不活动与活动的关系。静止期自发性运动性癫痫的发生率显著高于活动期(82%vs.18%，P=0.0001)。虽然光照期间癫痫发作多于黑暗时期，但差异并不显著(62%比38%，P>；0.1)。这些数据表明，在红藻氨酸诱导的癫痫大鼠中，自发运动发作的频率主要取决于活动状态，而不是一天中的时间(即光暗周期中的时间)。在红藻氨酸诱导的癫痫大鼠中，不活动对癫痫发生的影响似乎类似于某些形式的人类癫痫。(C)1999 Elsevier Science B.V.保留所有权利。
Kainate treatment in rats can result in a chronic behavioral state that is similar to human temporal lobe epilepsy. We tested the hypothesis that, like some humans with epilepsy, rat with kainate-induced epilepsy have more spontaneous motor seizures during inactivity (i.e. little to no volitional movement, including apparent sleep) than during activity (i.e. apparent volitional movement, as in walking, grooming, eating, etc.). Rats were given intraperitoneal (i.p.) injections of kainate (5 mg/kg) every hour so that class III/IV/V seizures were elicited for greater than or equal to 3 h. Seizure behavior was video-monitored (24 h for 5-6 days, n = 32 rats at 3 months and n = 23 rats at 4 months after treatment) to examine the occurrence of seizures as a function of light versus dark (12-12-h light-dark cycle) and inactivity versus activity. Significantly more spontaneous motor seizures occurred during inactive versus active states (82% vs. 18%, P = 0.0001). Although more seizures occurred during the light period than the dark, the difference was not significant (62% vs. 38%, P > 0.1). These data suggest that the frequency of spontaneous motor seizures in the rat with kainate-induced epilepsy depends primarily on activity state rather than time of day (i.e. time during the light-dark cycle). The effect of inactivity on the occurrence of seizures in the rat with kainate-induced epilepsy appears similar to some forms of human epilepsy. (C) 1999 Elsevier Science B.V. All rights reserved.