MULTIPLE CLASSES OF CATECHOLAMINE VESICLES OBSERVED DURING EXOCYTOSIS FROM THE PLANORBIS CELL BODY
MULTIPLE CLASSES OF CATECHOLAMINE VESICLES OBSERVED DURING EXOCYTOSIS FROM THE PLANORBIS CELL BODY
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DOI：
10.1016/0006-8993(95)00989-9
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发表时间：
1995-12-01
期刊：
影响因子：
2.9
通讯作者：
EWING, AG
中科院分区：
文献类型：
--
作者：
CHEN, GY;EWING, AG
囊泡的异质性已被研究，通过评估从含多巴胺的神经元的Planorbis corneus的细胞体释放的胞吐事件。囊泡胞吐已引发的刺激与原位应用的高钾，并已被电化学监测与碳纤维微电极放置在细胞体。这些电极允许监测和定量个体释放事件以揭示囊泡多巴胺含量。单个释放事件的统计分析表明，两类囊泡与特定的双峰分布在多巴胺含量和囊泡大小观察细胞刺激后。通过用D-安非他明处理细胞，研究了精神兴奋剂对个体囊泡多巴胺水平的影响。20分钟后，应用10 μ M安非他明，囊泡含量和大小分布的变化，获得与囊泡多巴胺水平的整体下降。的40%。改变的分布显示三峰形状，表明安非他明处理产生了第三类囊泡。我们的数据似乎表明，多个类别的囊泡从含多巴胺的神经元的细胞体中释放，并且囊泡多巴胺水平可以通过应用亲脂性弱碱性安非他明来操纵。
The heterogeneous nature of vesicles has been studied by evaluating exocytotic events released from the cell body of a dopamine-containing neuron of Planorbis corneus. Vesicular exocytosis has been elicitied by stimulation with in situ application of elevated potassium and has been monitored electrochemically with a carbon fiber microelectrode placed on the cell body. These electrodes allow individual release events to be monitored and quantitated to reveal vesicular dopamine content. Statistical analysis of individual release events demonstrates that two classes of vesicles with specific bimodal distributions in dopamine content and vesicle size are observed after cell stimulation. The effect of a psychostimulant on individual vesicular dopamine level has been studied by treating the cells with D-amphetamine. After a 20-min application of 10 mu M amphetamine, changes in both vesicle content and size distributions are obtained with an overall decrease in vesicular dopamine level. of 40%. The altered distribution show trimodal shapes indicating a third class of vesicles is created by amphetamine treatment. Our data appears to indicate that multiple classes of vesicles are released from the cell body of the dopamine-containing neuron and vesicular dopamine level can be manipulated by the application of the lipophilic weak base amphetamine.