A novel frameshift mutant allele, fzp-10, affecting the panicle architecture of rice
A novel frameshift mutant allele, fzp-10, affecting the panicle architecture of rice
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DOI：
10.1007/s10681-011-0547-2
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发表时间：
2012-03-01
期刊：
影响因子：
1.9
通讯作者：
Horibata, Akira
中科院分区：
文献类型：
--
作者：
Kato, Tsuneo;Horibata, Akira
水稻FRIZZY PANICLE（FZP）基因位于第7染色体上，该基因含有一个ERF和酸性结构域，与小穗分生组织特性的调节和穗构型的决定有关。许多fzp突变体显著改变了穗部形态，其花器官的发育与穗轴高位分枝的相继发育无关。用γ射线辐照水稻品种“金宝津”的种子，诱导出一个新的具有相同fzp表型的突变体。对该突变体与已知的fzp-1等位基因的等位性、核苷酸序列、穗部性状和农艺性状的分析表明，该突变体的等位基因位于FZP中。因为有一个先前鉴定的等位基因称为fzp-9，我们将这个新的等位基因命名为fzp-10。fzp-10在ERF结构域和酸性结构域之间具有单核苷酸（胞嘧啶）缺失，这导致移码突变和提前终止密码子，从而改变编码蛋白的C末端。与fzp-1相比，fzp-10突变体的穗中的高阶分枝的程度显著降低。此外，与野生型和fzp-1相比，fzp-10突变体的秆长、穗长和穗数显著降低，抽穗期延迟。fzp-10具有多个新的特性，其核苷酸位置的改变和表型改变的严重程度，因此，它可能成为研究FZP功能和决定水稻穗构型的新基因资源。
The rice FRIZZY PANICLE (FZP) locus on chromosome 7, in which an ERF and acidic domain are present, is concerned with the regulation of spikelet meristem identity and the determination of panicle architecture. Many fzp mutants drastically alter panicle morphology with higher-order rachis-branches developing successively instead of the development of floral organs in these mutants. A new mutant showing the same fzp phenotype was induced by gamma-ray irradiation of seeds of a rice cultivar "Gimbozu". Examination of this fzp-like mutant for its allelism to a known allele of fzp-1, nucleotide sequence, and panicle and agronomic characteristics clearly indicated that the allele of this fzp-like mutant is located in FZP. Because there is a previously identified allele called fzp-9, we designated this new allele as fzp-10. fzp-10 has a single nucleotide (cytosine) deletion between the ERF domain and the acidic domain, which results in a frameshift mutation and a premature stop codon, thereby altering the C-terminus of the encoded protein. The degree of higher-order branching in the panicles was significantly reduced in the fzp-10 mutant compared with that of fzp-1. Moreover, the fzp-10 mutant showed highly depressed culm and panicle lengths and panicle number, as well as delayed heading dates compared with its wild type and also with fzp-1. fzp-10 has several new characteristics, its altered nucleotide position and severity of phenotype alteration, and, therefore, could be a new gene resource to examine the function of FZP and the determination of rice panicle architecture.