Deterministic chaos and noise in three in vitro hippocampal models of epilepsy.
Deterministic chaos and noise in three in vitro hippocampal models of epilepsy.
复制标题
三种体外海马癫痫模型的确定性混沌和噪声。
DOI：
10.1114/1.1380419
复制
发表时间：
2001
影响因子：
3.8
通讯作者：
Mogul,DJ
中科院分区：
文献类型：
--
作者：
Slutzky,MW;Cvitanović,P;Mogul,DJ
最近的报告表明，混沌控制技术可能是有用的电操纵癫痫样的神经元群的爆发行为。由于自发性玻璃体爆发的动力学以前没有很好地确定，因此在大鼠海马中进行了这种行为的分析。采用三种实验方法在大鼠海马脑片上诱发癫痫样爆发。将切片浸泡在含有以下物质的人工脑脊液中：（1）高钾（[K+]o=10.5 mM），（2）零镁，或（3）GABAA受体拮抗剂荷包牡丹碱（20 μM）和印防己毒素（250 μM）。混沌和确定性的存在进行了评估，使用两种不同的分析技术：不稳定周期轨道（UPO）分析和一种新的技术估计李雅普诺夫指数。通过将每个实验的计算结果与相应的随机化替代数据进行比较，评估这些结果的显著性。在所有三种癫痫模型的实验中，多个时期的UPO非常普遍：所有实验中有73%包含至少一个具有统计学意义的时期1或时期2轨道。然而，膨胀率分析并没有提供数据中存在决定论的任何证据。这表明系统可能是全局随机的，但包含局部的确定性。因此，使用混沌控制算法操纵突发行为可能仍然有望恢复或预防癫痫发作。©2001 Biomedical Engineering Society.PAC01：8719Nn，8719Xx，0545Gg，8717Nn，8719La
Recent reports have suggested that chaos control techniques may be useful for electrically manipulating epileptiform bursting behavior in neuronal ensembles. Because the dynamics of spontaneousin vitrobursting had not been well determined previously, analysis of this behavior in the rat hippocampus was performed. Epileptiform bursting was induced in transverse rat hippocampal slices using three experimental methods. Slices were bathed in artificial cerebrospinal fluid containing: (1) elevated potassium ([K+]o=10.5 mM), (2) zero magnesium, or (3) the GABAA-receptor antagonists bicuculline (20 μM) and picrotoxin (250 μM). The existence of chaos and determinism was assessed using two different analytical techniques: unstable periodic orbit (UPO) analysis and a new technique for estimating Lyapunov exponents. Significance of these results was assessed by comparing the calculations for each experiment with corresponding randomized surrogate data. UPOs of multiple periods were highly prevalent in experiments from all three epilepsy models: 73% of all experiments contained at least one statistically significant period-1 or period-2 orbit. However, the expansion rate analysis did not provide any evidence of determinism in the data. This suggests that the system may be globally stochastic but contains local pockets of determinism. Thus, manipulation of bursting behavior using chaos control algorithms may yet hold promise for reverting or preventing epileptic seizures. ©2001 Biomedical Engineering Society.PAC01: 8719Nn, 8719Xx, 0545Gg, 8717Nn, 8719La