Simple and Sensitive Determination of Aromatic Acids in Coconut Water by g-C3N4@SiO2 Based Solid-phase Extraction and HPLC-UV Analysis
Simple and Sensitive Determination of Aromatic Acids in Coconut Water by g-C3N4@SiO2 Based Solid-phase Extraction and HPLC-UV Analysis
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基于 g-C3N4@SiO2 的固相萃取和 HPLC-UV 分析简单、灵敏地测定椰子水中的芳香酸
DOI：
10.1007/s40242-018-8041-1
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发表时间：
2018
影响因子：
3.1
通讯作者：
Feng Yu-Qi
中科院分区：
文献类型：
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作者：
Qiangba Gezhen;Zhu Gang-Tian;Yu Qiong-Wei;Feng Yu-Qi
本研究通过三聚氰胺在二氧化硅上的简单热解制备了石墨氮化碳沉积二氧化硅（g-C3N4@SiO2），然后将其用作固相萃取（SPE）吸附剂，用于萃取椰子水（CW）样品中的四种代表性芳香酸，包括苯甲酸（BA）、水杨酸（SA）、吲哚基-3-丁酸（IBA）和萘乙酸（NAA）。 g-C3N4@SiO2对浓度范围为500-558.8 μg/g的四种芳香酸表现出优异的吸附能力。 g-C3N4@SiO2 可以在 5 分钟内从 CW 样品中直接捕获四种芳香酸。因此，通过将g-C3N4@SiO2基SPE与高效液相色谱-紫外(HPLC-UV)检测相结合，开发了一种快速、简单、有效的CW样品中四种芳香酸的分析方法。该方法的线性范围为20-1000 ng/mL，检测限为1.9-5.7 ng/mL，显着低于已报道的同类方法。四种芳香酸的日内精密度和日间精密度（基于相对标准差，n=3）分别低于9.5%和10.4%。所开发的方法用于测定实际 CW 样品中的四种芳香酸，加标回收率在 81.1% 至 121.8% 之间。
In this study, graphitic carbon nitride deposited silica(g-C3N4@SiO2) was prepared by simple pyrolysis of melamine on silica and then used as a solid-phase extraction(SPE) sorbent for the extraction of four representative aromatic acids including benzoic acid(BA), salicylic acid(SA), indolyl-3-butyricacid(IBA) and naphthalene acetic acid(NAA) in coconut water(CW) samples. g-C3N4@SiO2exhibited an excellent adsorption capacity for the four aromatic acids, which are in the concentration range of 500–558.8 μg/g. The four aromatic acids could be directly captured by g-C3N4@SiO2from CW samples within 5 min. Thus, a rapid, simple and effective method for the analysis of four aromatic acids in CW samples was developed by coupling g-C3N4@SiO2-based SPE with high performance liquid chromatography-ultraviolet(HPLC-UV) detection. The linearity of the developed method was in the range of 20–1000 ng/mL and its limit of detection was in the range of 1.9–5.7 ng/mL, which were significantly lower than those of the reported similar methods. The intraday and interday precisions(based on the relative standard deviation,n=3) of the four aromatic acids were under 9.5% and 10.4%, respectively. The developed method was applied to determining the four aromatic acids in real CW samples and the spiked recoveries varied from 81.1% to 121.8%.