The glutamine synthetase gene family in Populus.
The glutamine synthetase gene family in Populus.
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Populus中的谷氨酰胺合成酶基因家族。
DOI：
10.1186/1471-2229-11-119
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发表时间：
2011-08-25
影响因子：
5.3
通讯作者：
Cánovas FM
中科院分区：
文献类型：
--
作者：
Castro-Rodríguez V;García-Gutiérrez A;Canales J;Avila C;Kirby EG;Cánovas FM
谷氨酰胺合成酶（GS；EC：6.3.1.2，L-谷氨酸：ADP形成氨连接酶）是高等植物铵同化和代谢的关键酶。目前的工作是为了更全面地了解杨树GS基因家族的分子和生化特征，并表征杨树多年生生长过程中不同组织和不同时期GS表达的发育调控。 GS 基因家族由 8 个不同的基因组成，表现出与其作为功能基因的作用一致的所有结构和调控元件。我们的结果表明，该家族成员由 4 组重复基因组成，其中 3 组编码胞质 GS 亚型（GS1），1 组编码脉络膜 GS 亚型（GS2）。我们的分析表明，毛果杨是第一个观察到完整 GS 家族复制的植物物种。详细的表达分析揭示了杨树中 GS 表达的特定空间和季节模式。这些数据提供了对杨树中 GS 异构体代谢功能的深入了解，并为未来的功能研究铺平了道路。我们的数据表明，可以保留 GS 重复，以增加特定细胞类型中的酶量。这种可能性可能有助于与谷氨酰胺衍生代谢产物变化相关的功能中氮代谢的稳态。杨树中重复的 GS 基因的存在也可能通过在特定细胞类型中将 GS 多肽组装成同源寡聚和/或异源寡聚全酶而有助于特定 GS 同工型的酶性质的多样化。
Glutamine synthetase (GS; EC: 6.3.1.2, L-glutamate: ammonia ligase ADP-forming) is a key enzyme in ammonium assimilation and metabolism of higher plants. The current work was undertaken to develop a more comprehensive understanding of molecular and biochemical features of GS gene family in poplar, and to characterize the developmental regulation of GS expression in various tissues and at various times during the poplar perennial growth. The GS gene family consists of 8 different genes exhibiting all structural and regulatory elements consistent with their roles as functional genes. Our results indicate that the family members are organized in 4 groups of duplicated genes, 3 of which code for cytosolic GS isoforms (GS1) and 1 which codes for the choroplastic GS isoform (GS2). Our analysis shows that Populus trichocarpa is the first plant species in which it was observed the complete GS family duplicated. Detailed expression analyses have revealed specific spatial and seasonal patterns of GS expression in poplar. These data provide insights into the metabolic function of GS isoforms in poplar and pave the way for future functional studies. Our data suggest that GS duplicates could have been retained in order to increase the amount of enzyme in a particular cell type. This possibility could contribute to the homeostasis of nitrogen metabolism in functions associated to changes in glutamine-derived metabolic products. The presence of duplicated GS genes in poplar could also contribute to diversification of the enzymatic properties for a particular GS isoform through the assembly of GS polypeptides into homo oligomeric and/or hetero oligomeric holoenzymes in specific cell types.
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影响因子：
10.7
作者：
Hanada K;Sawada Y;Kuromori T;Klausnitzer R;Saito K;Toyoda T;Shinozaki K;Li WH;Hirai MY
通讯作者：
Hirai MY
影响因子：
3.5
作者：
Canales, Javier;Flores-Monterrosso, Arantxa;Canovas, Francisco M.
通讯作者：
Canovas, Francisco M.
影响因子：
4.3
作者：
CANOVAS, FM;CANTON, FR;DEVICENTE, A
通讯作者：
DEVICENTE, A
影响因子：
7.2
作者：
Gómez-Maldonado, J;Avila, C;Campbell, MM
通讯作者：
Campbell, MM
影响因子：
4.3
作者：
Brugière, N;Dubois, F;Hirel, B
通讯作者：
Hirel, B