Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut.
Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut.
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DOI：
10.1021/jf4022509
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发表时间：
2014-01-08
影响因子：
6.1
通讯作者：
Wünschmann S
中科院分区：
文献类型：
--
作者：
Koid AE;Chapman MD;Hamilton RG;van Ree R;Versteeg SA;Dreskin SC;Koppelman SJ;Wünschmann S
The similarities of two major peanut allergens, Ara h 2 and Ara h 6, in molecular size, amino acid sequence, and structure have made it difficult to obtain natural Ara h 6 free of Ara h 2. The objectives of this study were to purify natural Ara h 6 that is essentially free of Ara h 2 and to compare its IgE reactivity and potency in histamine release assays to Ara h 2. SDS-PAGE of the highly purified allergen (<0.01% Ara h 2) revealed a single 14.5kD band and the identity of Ara h 6 was confirmed by LC-MS/MS. Ara h 6 showed a higher seroprevalence in chimeric-IgE ELISA (n=54), but a weaker biological activity in basophil histamine release assays than Ara h 2. Purified Ara h 6 will be useful for diagnostic IgE antibody assays, as well as molecular and cellular studies to investigate the immunological mechanisms of peanut allergy.
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