STUDIES ON THE NUTRITION AND PHYSIOLOGY OF PASTEURELLA-PESTIS .1. A CASEIN HYDROLYZATE MEDIUM FOR THE GROWTH OF PASTEURELLA-PESTIS
STUDIES ON THE NUTRITION AND PHYSIOLOGY OF PASTEURELLA-PESTIS .1. A CASEIN HYDROLYZATE MEDIUM FOR THE GROWTH OF PASTEURELLA-PESTIS
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DOI：
10.1128/jb.73.1.122-129.1957
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发表时间：
1957-01-01
影响因子：
3.2
通讯作者：
CARLIN, CE
中科院分区：
文献类型：
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作者：
HIGUCHI, K;CARLIN, CE
含有酪蛋白部分酸水解物、盐和碳水化合物的改进生长培养基在27 ℃下孵育时能够支持50-100 x 109个活细胞/ml。D-木糖和D-甘露糖醇都可用作培养基中的碳水化合物底物，但葡萄糖只能在特殊条件下使用，因为抑制量的有机酸的积累。建立了培养基中有机和无机成分的最佳浓度，该培养基适合所有菌株的良好生长。为了在37 ℃培养，培养基中补充甘氨酸和B族维生素，Mg++浓度增加. 37 ℃下的产率在20-30 × 109个细胞/ml的范围内.
An improved growth medium containing a partial acid hydroly-zate of casein, salts, and a carbohydrate is capable of supporting 50-100 x 109 viable cells/ml upon incubation at 27 C. D-xylose and D-mannitol were each useful as a carbohydrate substrate in the medium, but glucose may be used only under special conditions because of accumulation of inhibitory quantities of organic acids. Optimal concentrations of both organic and inorganic components of the medium were established and the medium was adequate for good growth of all strains. For culturing at 37 C, the medium is supplemented with glycine and B vitamins, and the Mg++ concentration increased. Yields at 37 C were in the range of 20-30 x 109 cells/ml.