Identification of a mosquitocidal toxin from Bacillus thuringiensis using mass spectrometry
Identification of a mosquitocidal toxin from Bacillus thuringiensis using mass spectrometry
复制标题
DOI：
10.1007/s11274-014-1744-7
复制
发表时间：
2014-09
影响因子：
4.1
通讯作者：
Wenfei Zhang;Jie Zhang;N. Crickmore;Zhongqi Wu;Yiran Yang;Jiangzhao Qian;Hongping Wu;Ruiping Wang;X. Fang
中科院分区：
文献类型：
--
作者：
Wenfei Zhang;Jie Zhang;N. Crickmore;Zhongqi Wu;Yiran Yang;Jiangzhao Qian;Hongping Wu;Ruiping Wang;X. Fang
苏云金芽孢杆菌菌株S2160-1先前已被鉴定为对蚊子幼虫具有高毒性，并且是目前商业上用于控制这些昆虫的菌株的可行替代品。通过PCR方法鉴定了该菌株的4个杀虫毒素基因（cry 30 Ea、cry 30 Ga、cry 50 Ba和cry 54 Ba），但未鉴定出编码140、130和30 kDa的3个主要晶体毒素蛋白的基因。在这项研究中，我们使用质谱鉴定130 kDa的毒素作为一种罕见的Cry 4毒素（Cry 4Cb 3）。对该毒素的编码基因进行了克隆和表达，并对致倦库蚊进行了杀虫活性测定。
TheBacillus thuringiensisstrain S2160-1 has previously been identified as being highly toxic to mosquito larvae and a viable alternative to strains currently used commercially to control these insects. A PCR approach had identified the presence of four putative insecticidal toxin genes (cry30Ea,cry30Ga,cry50Baandcry54Ba) in this strain, but did not identify the genes that encoding three of the main crystal toxin proteins of size 140 and 130 and 30 kDa. In this study we used mass spectrometry to identify the 130 kDa toxin as a rare Cry4 toxin (Cry4Cb3). The gene encoding this toxin was cloned and expressed and the toxin shown to have mosquitocidal activity againstCulex quinquefasciatus.