ALTERNATIVE MODES OF SELF-CLEAVAGE BY NEWT SATELLITE-2 TRANSCRIPTS
ALTERNATIVE MODES OF SELF-CLEAVAGE BY NEWT SATELLITE-2 TRANSCRIPTS
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DOI：
10.1093/nar/19.7.1699
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发表时间：
1991-04-11
影响因子：
14.9
通讯作者：
PABONPENA, LM
中科院分区：
文献类型：
--
作者：
EPSTEIN, LM;PABONPENA, LM
来自蝾螈的卫星 2 DNA 的合成转录本在体外经历自催化切割。在本报告中，我们提供的证据表明卫星 2 转录物自切割至少有两种不同的模式。在一种模式中，单个切割结构域折叠成以慢速切割的结构。这种结构可能类似于“锤头”结构，或者是“锤头”结构的变体，“锤头”结构被认为在许多感染性植物 RNA 的自我裂解过程中具有活性。在替代模式中，多个切割域相互作用以提高切割速率。重复卫星 2 序列的排列决定了这些切割模式中的哪一种将占主导地位，大概是通过影响 RNA 的整体构象。我们提出了一种多聚体卫星 2 转录物自我加工的模型，其中结合了这两种自我切割模式。
Synthetic transcripts of satellite 2 DNA from the newt undergo self-catalyzed cleavage in vitro. In this report we present evidence that there are at least two distinct modes of satellite 2 transcript self-cleavage. In one mode, a single cleavage domain folds into a structure which cleaves at a slow rate. This structure may be analogous to, or a variant of, the 'hammerhead' structure believed to be active during the self-cleavage of a number of infectious plant RNAs. In an alternative mode, multiple cleavage domains interact to cleave at an enhanced rate. The permutation of the repeated satellite 2 sequence determines which of these modes of cleavage will predominate, presumably by influencing the overall conformation of the RNA. We present a model for the self-processing of multimeric satellite 2 transcripts which incorporates both of these modes of self-cleavage.