Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase:: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase:: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
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DOI：
10.1128/ec.2.1.34-48.2003
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发表时间：
2003-02-01
期刊：
影响因子：
--
通讯作者：
Goldman, MHS
中科院分区：
文献类型：
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作者：
Goldman, GH;Marques, ED;Goldman, MHS
巴西拟球孢子菌是一种热变形真菌，是拉丁美洲流行的系统性真菌病--拟球孢子菌病的病原体。我们在这里提出了一个在酵母致病阶段表达的基因的调查。我们从5‘和3’端获得了13,490个表达序列标签。聚类分析得到了4692个表达基因的部分序列，这些基因根据与已知基因的相似性进行了功能分类。我们已经在巴西假丝酵母菌中鉴定了几种白念珠菌的毒力和致病同源物。此外，我们还利用实时定量逆转录-聚合酶链式反应分析了其中一些基因在酵母-菌丝体-酵母二态转变过程中的表达。菌丝体-酵母转变的聚类分析显示三个组：(I)RBT、疏水素和异柠檬酸裂解酶；(Ii)苹果酸脱氢酶、重叠群Pb1067和Pb1145、GPI和交替氧化酶；以及(Iii)泛素、增量-9-去饱和酶、HSP70、HSP82和Hsp104。前两组在菌丝期的mRNA表达较高，而第三组在酵母期的mRNA表达较高。我们的结果表明，可能保存了真菌之间的致病力和毒力机制，极大地扩展了巴西巴氏杆菌的基因鉴定，并为进一步了解其生物学特性提供了更广泛的基础。
Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis. We present here a survey of expressed genes in the yeast pathogenic phase of P. brasiliensis. We obtained 13,490 expressed sequence tags from both 5' and 3' ends. Clustering analysis yielded the partial sequences of 4,692 expressed genes that were functionally classified by similarity to known genes. We have identified several Candida albicans virulence and pathogenicity homologues in P. brasiliensis. Furthermore, we have analyzed the expression of some of these genes during the dimorphic yeast-mycelium-yeast transition by real-time quantitative reverse transcription-PCR. Clustering analysis of the mycelium-yeast transition revealed three groups: (i) RBT, hydrophobin, and isocitrate lyase; (ii) malate dehydrogenase, contigs Pb1067 and Pb1145, GPI, and alternative oxidase; and (iii) ubiquitin, delta-9-desaturase, HSP70, HSP82, and HSP104. The first two groups displayed high mRNA expression in the mycelial phase, whereas the third group showed higher mRNA expression in the yeast phase. Our results suggest the possible conservation of pathogenicity and virulence mechanisms among fungi, expand considerably gene identification in P. brasiliensis, and provide a broader basis for further progress in understanding its biological peculiarities.