Low level lead (Pb) exposure during gestation and lactation: assessment of effects on pubertal development in Fisher 344 and Sprague-Dawley female rats
Low level lead (Pb) exposure during gestation and lactation: assessment of effects on pubertal development in Fisher 344 and Sprague-Dawley female rats
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DOI：
10.1016/j.lfs.2003.07.033
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发表时间：
2004-01-16
期刊：
影响因子：
6.1
通讯作者：
Bratton, GR
中科院分区：
文献类型：
--
作者：
Dearth, RK;Hiney, JK;Bratton, GR
使用Fisher 344和Sprague-Dawley（SD）大鼠系的研究表明，妊娠期和/或哺乳期母体铅（Pb）暴露导致其各自雌性后代生殖成熟延迟。由于这些研究采用不同的实验方案的剂量和监测铅的水平，它一直无法确定哪种大鼠线提供了最佳的模型，低水平的铅毒性研究。本研究旨在解决这个问题。成年Fisher和SD雌性大鼠给予含有12 mg Pb/ml的PbAc溶液或乙酸钠（NaAc）作为对照。在繁殖前30天开始给药，并持续至幼仔21日龄断奶。在繁殖和断奶时，Fisher母鼠的血铅（BPb）水平平均为37.3 mug/dl，SD母鼠平均为29.9 mug/ dl。铅使Fisher子代青春期延迟（p < 0.01），血清促黄体生成素（LH，p < 0.001）和雌二醇（E-2，p < 0.01）水平降低。这些影响在SD后代中未发生。将给予SD大鼠的剂量加倍，使其BPb水平增加至62.6 μg/dl，但仍然没有观察到任何影响。这些结果表明，费舍尔后代更敏感的母体铅暴露青春期相关的侮辱比SD大鼠，这表明费舍尔线可能是一个更可靠的啮齿动物模型，以研究低水平的铅毒性的影响。(C)2003年爱思唯尔公司All rights reserved.
Studies using both Fisher 344 and Sprague-Dawley (SD) rat lines have shown that gestational and/or lactational maternal lead (Pb) exposure causes delayed reproductive maturation in their respective female offspring. Because these studies utilized different experimental regimens for dosing and for monitoring Pb levels, it has not been possible to determine which rat line provides the best model for low level Pb toxicity studies. This study was designed to address this issue. Adult Fisher and SD female rats were dosed with either a solution of PbAc containing 12 mg of Pb/ml or sodium acetate (NaAc) for controls. Dosing began 30 days prior to breeding and continued until their pups were weaned at 21 days of age. At the time of breeding and through weaning the blood lead (BPb) levels in the Fisher dams averaged 37.3 mug/dl and the SD dams averaged 29.9 mug/ dl. Pb delayed the timing of puberty (p < 0.01) in Fisher offspring, and suppressed serum levels of luteinizing hormone (LH, p < 0.001) and estradiol (E-2, p < 0.01). These effects did not occur in the SD offspring. Doubling the dose given to the SD rats increased their BPb levels to 62.6 μg/dl, yet there were still no effects noted. These results indicate that Fisher offspring are more sensitive to maternal Pb exposure with regard to puberty related insults than are SD rats, suggesting that the Fisher line may be a more reliable rodent model to study the effects of low level Pb toxicity. (C) 2003 Elsevier Inc. All rights reserved.