Prevalence of Nosema species infections in Apis cerana japonica and Apis mellifera honeybees in the Tohoku region of Japan
Prevalence of Nosema species infections in Apis cerana japonica and Apis mellifera honeybees in the Tohoku region of Japan
复制标题
日本东北地区中华蜜蜂和意大利蜜蜂中微孢子虫感染的流行情况
DOI：
10.1016/j.parint.2021.102361
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发表时间：
2021
影响因子：
1.9
通讯作者：
Tadashi Itagaki
中科院分区：
文献类型：
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作者：
Shunsuke Takashima;Yuma Ohari;Tadashi Itagaki
我们调查了日本东北地区中华蜜蜂和意大利蜜蜂微孢子虫感染的流行情况。在509A中，有14例（2.8%）检出了微孢子虫DNA。cerana japonica和155A.从青森、岩手、秋田、山形、福岛等县的蜜蜂中检测到微孢子虫。cerana japonica和A.意利费拉。在遗传上与N. apis，新单胞菌N. cerebral，和N.根据小亚基（SSU）rDNA，大亚基rDNA和内转录间隔区序列，在105个（20.6%）的509A中鉴定出了大肠杆菌。cerana japonica和155A. melliferahoneybees和岩手县。基于SSU rDNA序列的系统发育树显示微孢子虫属。与N属于同一进化枝。thomsonisdetected在蛾和孤独的蜜蜂在北美和N.在土耳其的卷心菜蝴蝶中发现了pieriaa，迄今为止还没有在蜜蜂中发现。微孢子虫的种属鉴定需要对孢子形态特征进行分析。
We investigated here, the prevalence ofNosemamicrosporidia infections in the honeybees,Apis ceranajaponicaandApis mellifera, in the Tohoku region of Japan. We detectedNosema ceranaeDNA in 14 (2.8%) of 509A. cerana japonicaand in 34 (21.9%) of 155A. melliferahoneybees from Aomori, Iwate, Akita, Yamagata, and Fukushima prefectures.NosemaapisDNA was undetectable inA. cerana japonicaandA. mellifera. The unidentifiableNosemaspecies that genetically differed fromN. apis,N. ceranae, andN. neumanniin terms of small subunit (SSU) rDNA, large subunit rDNA, and internal transcribed spacer sequences was identified in 105 (20.6%) of 509A. cerana japonicaand in 1 (0.6%) of 155A. melliferahoneybees, and from Iwate prefecture. A phylogenetic tree based on SSU rDNA sequences showed that theNosemasp. belonged to the same clade asN. thomsonidetected in moth and solitary bees in North America andN. pieriaefound in cabbage butterfly in Turkey, which have not hitherto been detected in honeybees. The morphological characteristics of the spores should be analyzed to enable species identification of theNosemasp.