miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary
miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary
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miR-7介导NE信号通路影响猪垂体FSH和LH合成。
DOI：
10.1530/joe-19-0331
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发表时间：
2020-03-01
影响因子：
4
通讯作者：
Cui，Sheng
中科院分区：
文献类型：
--
作者：
Li，Xin;Li，Hongjiao;Cui，Sheng
microRNA-7（miR-7）在猪垂体中高表达，是多种基因表达及相关生物学过程的重要调控因子。去甲肾上腺素（NE）作为一种重要的神经递质或/和应激时过度分泌的激素，影响垂体促卵泡激素（FSH）和促黄体生成激素（LH）等调节性成熟和生殖功能的关键激素的合成和分泌。然而，NE，miR-7和促性腺激素之间的关系需要阐明。本研究的目的是鉴定miR-7是否参与NE-肾上腺素能受体信号通路影响猪垂体FSH和LH的合成和分泌。结果表明，侧脑室注射NE可增加猪垂体miR-7水平及FSH和LH的合成和分泌，而抑制内源性miR-7或β-肾上腺素能受体可抑制NE诱导的猪垂体前叶细胞FSH和LH合成的增加。进一步鉴定了猪垂体β-肾上腺素能受体的分子类型和信号通路，发现NE通过β 2肾上腺素能受体（β2AR）和RAF/MEK/ERK 1/2信号通路发挥作用。ERK 1/2的磷酸化上调miR-7水平，随后通过靶向高尔基体糖蛋白1（GLG 1）增强FSH和LH合成。这表明miR-7介导NE促进猪垂体FSH和LH合成的作用。
MicroRNA-7 (miR-7) is an important modulator of plenty gene expressions and the interrelated biological processes, highly expressed in porcine pituitary. Norepinephrine (NE), acting as an important neurotransmitter or/and a hormone secreted excessively under stress, affects the synthesis and secretion of various hormones, including pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which are the key hormones regulate sexual maturation and reproductive functions. However, the relationship among NE, miR-7 and gonadotropin needs to be elucidated. The aim of this study was to identify whether miR-7 involved in the NE-adrenoceptor signaling pathway affecting the synthesis and secretion of FSH and LH in porcine pituitary. Our results showed that the NE intracerebroventricular injection increased pituitary miR-7 level and the synthesis and secretion of FSH and LH in porcine, whereas the inhibition of either endogenous miR-7 or β-adrenergic receptors hindered the rise of FSH and LH synthesis induced by NE in cultured primary porcine anterior pituitary cells. Further, we identified the molecular type of β-adrenergic receptors and the signaling pathway in porcine pituitary, and we found that NE played its roles relying on adrenoceptor beta 2 (β2AR) and the RAF/MEK/ERK1/2 signaling pathway. The phosphorylation of ERK1/2 up-regulated miR-7 level which subsequently enhanced FSH and LH synthesis by targeting to Golgi glycoprotein 1 (GLG1). These suggest that miR-7 mediates NE's effect on promoting FSH and LH synthesis in porcine pituitary.