New “Ex Vivo” Radioisotopic Method of Quantitation of Platelet Deposition – Studies in Four Animal Species
New “Ex Vivo” Radioisotopic Method of Quantitation of Platelet Deposition – Studies in Four Animal Species
复制标题
血小板沉积定量的新“离体”放射性同位素方法 – 对四种动物物种的研究
DOI：
10.1055/s-0038-1665276
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发表时间：
1983
影响因子：
6.7
通讯作者：
M. Dewanjee
中科院分区：
文献类型：
--
作者：
L. Badimón;V. Fuster;J. Chesebro;M. Dewanjee
总结我们已经开发了一种灵敏的和定量的方法，“离体”评价血小板沉积在胶原条上，从兔跟腱，用流动的血液灌流，并将其应用于四种动物，猫，兔，狗和猪。自体血小板用铟-111-托酚酮标记，在灌注前24小时注射到动物体内，并从肌腱γ-辐射和血小板计数中定量沉积的血小板数量。当血液在胶原蛋白接触后再输注进入对侧颈静脉时，我们检测到一些血小板消耗与灌注时间相关，但如果血液在接触后被丢弃，则没有。因此，为了获得更符合生理学的动物模型，我们决定在肌腱灌注后弃血。在所有物种中，除猫外，肌腱上血小板的增加与血液灌流的暴露时间呈线性关系。胶原蛋白上沉积的血小板数量最多的是猫，最低的是狗。超微结构分析表明，血小板沉积后，只有5分钟的灌流聚集。
Summary We have developed a sensitive and quantitative method of “ex vivo” evaluation of platelet deposition on collagen strips, from rabbit Achilles tendon, superfused by flowing blood and applied it to four animal species, cat, rabbit, dog and pig. Autologous platelets were labeled with indium-111-tropolone, injected to the animal 24 hr before the superfusion and the number of deposited platelets was quantitated from the tendon gamma-radiation and the blood platelet count. We detected some platelet consumption with superfusion time when blood was reinfused entering the contralateral jugular vein after collagen contact but not if blood was discarded after the contact. Therefore, in order to have a more physiological animal model we decided to discard blood after superfusion of the tendon. In all species except for the cat there was a linear relationship between increase of platelet on the tendon and time of exposure to blood superfusion. The highest number of platelets deposited on the collagen was found in cats, the lowest in dogs. Ultrastructural analysis showed the platelets were deposited as aggregates after only 5 min of superfusion.