SWAP-70 is important for invasive phenotypes of mouse embryo fibroblasts transformed by v-src
SWAP-70 is important for invasive phenotypes of mouse embryo fibroblasts transformed by v-src
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DOI：
10.1002/iub.33
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发表时间：
2008-04-01
期刊：
影响因子：
4.6
通讯作者：
Fukui, Yasuhisa
中科院分区：
文献类型：
--
作者：
Murugan, Avaniyapuram Kannan;Ihara, Sayoko;Fukui, Yasuhisa
SWAP-70 是一种参与肌动蛋白重排的蛋白质，尤其是在膜褶皱中。缺乏 SWAP-70 的小鼠胚胎成纤维细胞 (MEF) 表现出膜皱褶受损，并且在 v-Src 转化后无法在软琼脂中生长。在这里，我们表明表达 SWAP-70 的 v-Src 转化 MEF 具有高度侵入性。单独表达 SWAP-70 或 v-Src 的 MEF 的侵袭性要小得多，这表明这两种蛋白都是细胞侵袭性所必需的。 SWAP-70 和 v-Src 的表达诱导持续的膜皱褶，这可能导致剧烈的细胞运动，这可能是细胞侵袭性所必需的。单独表达 v-Src 会在形态上改变 MEF，但形成片状伪足而不是膜皱褶，这表明与同时表达 SWAP-70 和 v-Src 的细胞相比，细胞的攻击性较低。这些结果表明v-Src和SWAP-70在MEF的侵袭活性中协同作用。 (c) 2008 年 IUBMB。
SWAP-70 is a protein involved in actin rearrangement, especially in membrane ruffling. Mouse embryo fibroblasts (MEFs) deficient in SWAP-70 show impaired membrane ruffling and fail to grow in soft agar after transformation by v-Src. Here, we show that v-Src transformed MEFs expressing SWAP-70 are highly invasive. MEFs expressing SWAP-70 or v-Src alone were far less invasive, suggesting that both proteins were required for the cells to be invasive. Expression of both SWAP-70 and v-Src induced constant membrane ruffling, which may cause vigorous cell movement, probably required for invasiveness of the cells. Expression of v-Src alone morphologically transformed MEFs but formed lamellipodia rather than membrane ruffles, suggesting less aggressive nature of the cells compared with those expressing both SWAP-70 and v-Src. These results suggest that v-Src and SWAP-70 act synergistically in the invasion activity of MEFs. (c) 2008 IUBMB.