Neuronal RNA oxidation is a prominent feature of dementia with Lewy bodies
Neuronal RNA oxidation is a prominent feature of dementia with Lewy bodies
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DOI：
10.1097/00001756-200211150-00009
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发表时间：
2002-11-15
期刊：
影响因子：
1.7
通讯作者：
Perry, G
中科院分区：
文献类型：
--
作者：
Nunomura, A;Chiba, S;Perry, G
用原位方法对路易体痴呆患者脑内氧化核苷8-羟基鸟苷进行了鉴定。胞浆内8-羟基鸟苷免疫反应明显的神经元广泛分布于海马区和颞叶新皮质。神经元8-羟基鸟苷免疫反应的相对强度测量显示，与对照组相比，路易体痴呆患者的核酸氧化明显增加。免疫染色前用核酸酶(DNase或RNase)处理表明，RNA是核酸氧化的主要部位。与先前报道的阿尔茨海默病和帕金森病易损神经元的RNA氧化一起，路易体痴呆的神经元RNA氧化可能是年龄相关性神经退行性疾病的基本异常之一。
An in situ approach was used to identify the oxidized nucleoside, 8-hydroxyguanosine in brains of dementia with Lewy bodies. Neurons with marked immunoreaction of 8-hydroxyguanosine in the cytoplasm were widely distributed in the hippocampal region and temporal neocortex. Relative intensity measurements of neuronal 8-hydroxyguanosine immunoreactivity showed that there was a significant increase in nucleic acid oxidation in dementia with Lewy bodies compared with controls. Treatment with nuclease (DNase or RNase) before the immunostaining demonstrated that RNA was a major site of nucleic acid oxidation. Together with the previously reported RNA oxidation in vulnerable neurons in Alzheimer and Parkinson diseases, neuronal RNA oxidation in dementia with Lewy bodies might represent one of the fundamental abnormalities in age-associated neurodegenerative diseases.