DETECTION OF HEPATITIS-A ANTIGEN IN HUMAN-LIVER
DETECTION OF HEPATITIS-A ANTIGEN IN HUMAN-LIVER
复制标题
DOI：
10.1128/iai.36.1.320-324.1982
复制
发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
TANIKAWA, K
中科院分区：
文献类型：
--
作者：
SHIMIZU, YK;SHIKATA, T;TANIKAWA, K
本文首次应用过氧化物酶-抗体技术，在光镜和电镜下，从一例甲型肝炎急性期患者肝活检组织中发现了甲型肝炎病毒抗原（HAAg）。在光学显微镜下，HAAg染色表现为细颗粒状反应产物，分散在肝细胞和肝窦衬里细胞的细胞质中。标准薄切片电镜显示肝细胞和枯否细胞胞质囊泡中有直径24-27 nm的病毒样颗粒。通过免疫过氧化物酶EM，在肝细胞胞质囊泡内聚集的颗粒上检测到HAAg，从而证明病毒样颗粒（24-27 nm）是甲型肝炎病毒。囊泡周围膜也呈HAAg阳性。HAAg在人肝脏中的分布模式与实验感染的绒猴的分布模式几乎相同。大多数HAAg在肝细胞胞质的囊泡内检测到，提示甲型肝炎病毒的囊泡定向形态发生的可能性。
For the 1st time, hepatitis A viral antigen (HAAg) was shown in liver biopsy tissue from a patient in the acute phase of hepatitis type A by light microscopy and EM, using the peroxidase-antibody technique. Under light microscopy, the staining for HAAg appeared as a fine, granular reaction product, scattered throughout the cytoplasm of hepatocytes and sinusoidal lining cells. Standard thin-section EM revealed virus-like particles, 24-27 nm in diameter, in cytoplasmic vesicles of hepatocytes and Kupffer cells. By immuno-peroxidase EM, HAAg was detected on particles aggregated within cytoplasmic vesicles of hepatocytes, thus demonstrating that the virus-like particles (24-27 nm) are hepatitis A virus. The surrounding membrane of the vesicles was also positive for HAAg. The distribution patterns of HAAg in human liver were virtually identical to those described for experimentally infected marmosets. Most HAAg was detected within vesicles of liver cell cytoplasm, suggesting the possibility of vesicle-oriented morphogenesis of hepatitis A virus.