Impact of transit time on the reproductive capacity of Euprymna scolopes as a laboratory animal.
Impact of transit time on the reproductive capacity of Euprymna scolopes as a laboratory animal.
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DOI：
10.1186/s42826-022-00135-2
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发表时间：
2022-07-30
影响因子：
2.9
通讯作者：
Miyashiro, Tim I.
中科院分区：
文献类型：
--
作者：
Cecere, Andrew G.;Miyashiro, Tim I.
夏威夷短尾鱿鱼（Euprymna scolopes）拥有各种海洋细菌共生体，这些共生体已经成为动物-微生物关系的模型，这对宿主的健康很重要。在一个发光的器官里，E. scolopes蕴藏着大量的费氏弧菌（Vibrio fischeri）细菌，这种细菌能产生低水平的生物发光，鱿鱼用它来伪装。这种共生关系最初是在幼乌贼从卵中孵化出来并从周围的海洋环境中获得细菌共生体后建立的。在海水养殖设施中相对容易地维持一群野生捕获的鱿鱼，这促进了30多年来有关幼鱿鱼的研究。然而，由于E. scolopes原产于夏威夷群岛，它们从夏威夷运到研究机构通常代表着一种压力，这可能会影响它们的生理机能。在这里，我们描述了动物的生存和生殖能力与一群鱿鱼聚集在两个运输明显不同的运输时间。我们发现，运输时间较长的动物产生的幼乌贼数量较低，不是由于运输的差异，而是由于产卵率较高的雌性乌贼数量较少，我们称之为高繁殖率。我们发现，繁殖能力极强的雌性占产卵总量的58%。本文讨论了这些发现对猪头足类动物的生物学和畜牧业的意义，从而为猪头足类动物作为一种有价值的微生物学实验动物的进一步研究和开发提供了一个平台。
The Hawaiian bobtail squid Euprymna scolopes hosts various marine bacterial symbionts, and these symbioses have served as models for the animal-microbe relationships that are important for host health. Within a light organ, E. scolopes harbors populations of the bacterium Vibrio fischeri, which produce low levels of bioluminescence that the squid uses for camouflage. The symbiosis is initially established after a juvenile squid hatches from its egg and acquires bacterial symbionts from the ambient marine environment. The relative ease with which a cohort of wild-caught E. scolopes can be maintained in a mariculture facility has facilitated over 3 decades of research involving juvenile squid. However, because E. scolopes is native to the Hawaiian archipelago, their transport from Hawaii to research facilities often represents a stress that has the potential to impact their physiology. Here, we describe animal survival and reproductive capacity associated with a cohort of squid assembled from two shipments with markedly different transit times. We found that the lower juvenile squid counts generated by animals with the longer transit time were not due to the discrepancy in shipment but instead to fewer female squid that produced egg clutches at an elevated rate, which we term hyper-reproductivity. We find that hyper-reproductive females were responsible for 58% of the egg clutches laid. The significance of these findings for E. scolopes biology and husbandry is discussed, thereby providing a platform for future investigation and further development of this cephalopod as a valuable lab animal for microbiology research.