Surveying Saccharomyces genomes to identify functional elements by comparative DNA sequence analysis
Surveying Saccharomyces genomes to identify functional elements by comparative DNA sequence analysis
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DOI：
10.1101/gr.182901
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发表时间：
2001-07-01
期刊：
影响因子：
7
通讯作者：
Johnston, M
中科院分区：
文献类型：
--
作者：
Cliften, PF;Hillier, LW;Johnston, M
比较序列分析促进了蛋白质编码基因和蛋白质内重要功能序列的发现，但对鉴定非蛋白质编码DNA中的功能序列元件的作用不大，因为非蛋白质编码序列的变化速度相对较快，非编码调控序列元件相对简单，需要对相对密切相关的物种进行序列比较。我们测试了比较DNA序列分析的使用，以帮助鉴定启动子调控元件，非蛋白编码RNA基因和小蛋白编码基因，通过调查几个Saccharomyces酵母物种的随机DNA序列，以了解哪些物种最适合与酿酒酵母进行比较。我们还确定了几种酵母菌物种的一些特定启动子和RNA基因的DNA序列，以确定基因组中已知功能元件的保守程度。我们的研究结果使我们得出结论，比较DNA序列分析将能够识别酵母基因组中功能保守的元素，并为获得这些信息提供了一条途径。
Comparative sequence analysis has facilitated the discovery of protein coding genes and important functional sequences within proteins, but has been less useful for identifying functional sequence elements in nonprotein-coding DNA because the relatively rapid rate of change of nonprotein-coding sequences and the relative simplicity of non-coding regulatory sequence elements necessitates the comparison of sequences of relatively closely related species. We tested the use of comparative DNA sequence analysis to aid identification of promoter regulatory elements, nonprotein-coding RNA genes, and small protein-coding genes by surveying random DNA sequences of several Saccharomyces yeast species, with the goal of learning which species are best suited for comparisons with S. cerevisiae. We also determined the DNA sequence of a few specific promoters and RNA genes of several Saccharomyces species to determine the degree of conservation of known Functional elements within the genome. Our results lead us to conclude that comparative DNA sequence analysis will enable identification of functionally conserved elements within the yeast genome, and suggest a path for obtaining this information.