The isolation of Leishmania tropica and L. aethiopica from Phlebotomus (Paraphlebotomus) species (Diptera: Psychodidae) in the Awash Valley, northeastern Ethiopia
The isolation of Leishmania tropica and L. aethiopica from Phlebotomus (Paraphlebotomus) species (Diptera: Psychodidae) in the Awash Valley, northeastern Ethiopia
复制标题
DOI：
10.1016/s0035-9203(03)00008-7
复制
发表时间：
2004-01-01
影响因子：
2.2
通讯作者：
Gramiccia, M
中科院分区：
文献类型：
--
作者：
Gebre-Michael, T;Balkew, M;Gramiccia, M
在阿瓦什山谷一个高度怀疑利什曼病的疫源地，（埃塞俄比亚东北部）在1994年1月至1997年8月期间，共有11种白蛉3307只雌性（P. orientalis、P. fantalensis、P. saevus、P. sergenti、P. gemetchi、P. alexandri、P. bergeroti、P. duboscqi、P. arabicus、P. martini和P. rodhaini）进行解剖。在3种鱼的17只雌鱼中检测到前鞭毛体，其中11只为萨氏前鞭毛体，4只为瑟氏前鞭毛体，2只为阿拉伯前鞭毛体。其中，只有两个P. soevus（一个来自上阿瓦什，一个来自中阿瓦什）和三个P. sergenti（来自上阿瓦什）阳性被成功分离培养，并通过同工酶分析分型。经鉴定，有4个菌株（分别来自P. saevus和P. sergenti）为L. tropica和1株来自P. sergenti的菌株被鉴定为L. aethiopica。这是首次发现美洲驼和阿拉伯驼自然感染，也是第一个证明美洲驼是埃塞俄比亚驼媒介的证据。热带。这也是首次将P. sergenti与L.在埃塞俄比亚的热带传播; L.另一新记录种为拟白蛉属Paraphlebotomus sergenti。可能存在的人类皮肤利什曼病（L。tropica和L. aethiopica），以及寄生虫的野生储存宿主，特别是阿瓦什河谷上游和中部的岩蹄兔（Procavia capensis），仍有待确定。(C)2003年皇家热带医学和卫生学会。出版社：Elsevier Ltd. rights reserved.
In a survey of Leishmania infections in phlebotomine sandflies in a highly suspected focus of leishmaniasis in the Awash Valley (northeastern Ethiopia) between January 1994 and August 1997, a total of 3307 females of 11 Phlebotomus species (P. orientalis, P. fantalensis, P. saevus, P. sergenti, P. gemetchi, P. alexandri, P. bergeroti, P. duboscqi, P. arabicus, P. martini, and P. rodhaini) were dissected. Promastigotes were detected in 17 females of three species (11 P. saevus, 4 P. sergenti and 2 P. arabicus). Of these, only two P. soevus (one from Upper Awash and one from Middle Awash) and three P. sergenti (from Upper Awash) positives were successfully isolated in culture and were typed by isoenzyme analysis. Four isolates (two each from P. saevus and P. sergenti) were identified as new zymodemes (Z) of L. tropica and one isolate from P. sergenti was typed as a new zymodeme of L. aethiopica.This is the first finding of natural infections of P saevus and P arabicus and the first evidence for the former to be a vector of L. tropica. This is also the first time P. sergenti has been implicated in L. tropica transmission in Ethiopia; the isolation of L. aethiopica from a Paraphlebotomus species (P. sergenti) is also a new record. The possible presence of human cutaneous leishmaniasis (L. tropica and L. aethiopica), and wild reservoir host(s) of the parasites, especially rock hyrax (Procavia capensis) in the Upper and Middle Awash Valley remain to be determined. (C) 2003 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All. rights reserved.