Persistent and Transient Airborne MRSA Colonization of Piglets in a Newly Established Animal Model.
Persistent and Transient Airborne MRSA Colonization of Piglets in a Newly Established Animal Model.
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DOI：
10.3389/fmicb.2018.01542
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发表时间：
2018
影响因子：
5.2
通讯作者：
Friese A
中科院分区：
文献类型：
--
作者：
Rosen K;Roesler U;Merle R;Friese A
家畜相关耐甲氧西林金黄色葡萄球菌（LA-MRSA）于2005年首次发现，迄今为止在畜牧业水库中广泛存在，重点是养猪业。在猪圈空气中以及猪场废气中MRSA的常规检测能力（平均计数：102 cfu/m3）提出了一个问题，即是否存在空气传播，因此是否存在通过空气传播途径的动物MRSA定植。为了回答这个问题，我们将三组9只MRSA阴性试验仔猪暴露于气溶胶室中规定的空气MRSA浓度（102、104和106 cfu/m3）24 h。在接下来的21天观察期内，通过采集不同拭子样本（鼻、咽、皮肤、结膜和直肠拭子）监测仔猪的MRSA状态。在实验结束时，我们对仔猪实施安乐死，并研究了MRSA在不同组织和器官中的传播。我们的研究数据表明存在空气传播的MRSA定植途径：暴露于空气中106 cfu/m3 MRSA的动物持续定植。暴露于104 cfu/m3空气中MRSA浓度的仔猪是短暂的，暴露于102 cfu/m3空气中MRSA浓度的仔猪没有定植。因此，通过空中路线的殖民化被证明。
Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) was first found in 2005 and is up to date widespread in animal husbandry reservoir – focusing on pig farming. The regular detectability of MRSA in the air of pigsties as well as in exhaust air of pig farms (mean count: 102 cfu/m3) poses the question whether an airborne spread and, therefore, a MRSA colonization of animals via the airborne route exists. To answer this question, we exposed three groups of nine MRSA-negative tested piglets each to a defined airborne MRSA concentration (102, 104, and 106 cfu/m3) in our aerosol chamber for 24 h. In the following observation period of 21 days, the MRSA status of the piglets was monitored by taking different swab samples (nasal, pharyngeal, skin, conjunctival, and rectal swab). At the end of the experiment, we euthanized the piglets and investigated different tissues and organs for the spread of MRSA. The data of our study imply the presence of an airborne MRSA colonization route: the animals exposed to 106 cfu/m3 MRSA in the air were persistent colonized. The piglets exposed to an airborne MRSA concentration of 104 cfu/m3 were transient, and the piglets exposed to an airborne MRSA concentration of 102 cfu/m3 were not colonized. Consequently, a colonization via the airborne route was proven.