Generation of an estrogen receptor beta-iCre knock-in mouse.
Generation of an estrogen receptor beta-iCre knock-in mouse.
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产生雌激素受体β-icre敲入小鼠。
DOI：
10.1002/dvg.22911
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发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
Ko C
中科院分区：
文献类型：
--
作者：
Cacioppo JA;Koo Y;Lin PC;Osmulski SA;Ko CD;Ko C
一种新型敲入小鼠，在雌激素受体β (Esr2)启动子的调控下表达密码子改良的Cre重组酶（iCre），用于条件缺失基因和Esr2表达的空间和/或时间定位。ESR2是两种经典的核雌激素受体之一，具有不同于另一种雌激素受体ESR1的时空表达模式和功能。构建了包含iCre、聚腺苷酸化序列和新霉素选择标记的卡带。该构建体通过同源重组将iCre插入C57BL/6J胚胎干细胞Esr2基因内源性起始密码子前。将得到的Esr2-iCre小鼠与ROSA26-lacZ和Ai9-RFP报告小鼠杂交，观察iCre功能表达的细胞。卵巢、垂体、睾丸间质、附睾头尾体、骨骼肌、凝血腺（前列腺前）、肺、包皮腺均有强表达。在大脑、下丘脑、心脏、肾上腺、结肠、膀胱和脚掌中也观察到弥漫性或斑片状表达。总的来说，ESR2 - icre小鼠将作为一种新的细胞系，在ESR2表达组织中有条件地消融基因，鉴定新的ESR2表达细胞，并区分ESR2和ESR1的功能。
A novel knock-in mouse that expresses codon-improved Cre recombinase (iCre) under regulation of the estrogen receptor beta (Esr2) promoter was developed for conditional deletion of genes and for the spatial and/or temporal localization of Esr2 expression. ESR2 is one of two classical nuclear estrogen receptors and displays a spatio-temporal expression pattern and functions that are different from the other estrogen receptor, ESR1. A cassette was constructed that contained iCre, a polyadenylation sequence, and a neomycin selection marker. This construct was used to insert iCre in front of the endogenous start codon of the Esr2 gene of a C57BL/6J embryonic stem cell line via homologous recombination. Resulting Esr2-iCre mice were bred with ROSA26-lacZ and Ai9-RFP reporter mice to visualize cells of functional iCre expression. Strong expression was observed in the ovary, the pituitary, the interstitium of the testes, the head and tail but not body of the epididymis, skeletal muscle, the coagulation gland (anterior prostate), the lung, and the preputial gland. Additional diffuse or patchy expression was observed in the cerebrum, the hypothalamus, the heart, the adrenal gland, the colon, the bladder, and the pads of the paws. Overall, Esr2-iCre mice will serve as a novel line for conditionally ablating genes in Esr2-expressing tissues, identifying novel Esr2-expressing cells, and differentiating the functions of ESR2 and ESR1.