Regulation of LH beta subunit mRNA in the sheep pituitary gland during different feedback states of estradiol.
Regulation of LH beta subunit mRNA in the sheep pituitary gland during different feedback states of estradiol.
复制标题
雌二醇不同反馈状态下绵羊垂体 LH β 亚基 mRNA 的调节
DOI：
10.1016/0006-291x(84)91234-8
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Kepa,J
中科院分区：
文献类型：
--
作者：
Landefeld,T;Kepa,J
用无细胞法检测了性腺类固醇激素对体外翻译的促黄体生成素（LH）β亚基的反馈作用。然后将这些量与各种反馈状态下的血清和垂体浓度相关联。通过免疫沉淀和凝胶电泳制备、翻译RNA并鉴定产物。β亚基的量以类似于α亚基观察到的模式变化。在切除卵巢的母羊中，β的量是负反馈组中观察到的量的2- 3倍，并且略高于表现出LH激增的动物中观察到的量。卵巢切除母羊垂体LH浓度也高于其他组，但正反馈组血清浓度最高。这些结果提供了证据：1）一个单独的，但协调，控制促性腺激素亚基的合成;和2）亚基合成的贡献，积极和消极的类固醇反馈垂体LH量的影响。
The feedback effects of gonadal steroids on the amounts ofin vitrotranslated luteinizing hormone (LH) beta subunit were examined using cell-free assays. These amounts were then correlated with serum and pituitary concentrations during various feedback states. RNA was prepared, translated and products identified by immunoprecipitation and gel electrophoresis. The amounts of beta subunit varied in a pattern similar to that observed for alpha subunit. In ovariectomized ewes, the amounts of beta were 2–3X those seen in negative feedback groups and slightly more than those seen in animals exhibiting an LH surge. The pituitary LH concentration in ovariectomized ewes was also higher than those seen in the other groups, however, the serum concentrations in the positive feedback group were the highest of all groups. These results provide evidence for: 1) a separate, but coordinate, control of gonadotropin subunit synthesis; and 2) a contribution of subunit synthesis to the effects of positive and negative steroid feedback on pituitary LH amounts.