Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed ( Striga hermonthica )
Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed ( Striga hermonthica )
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寄生植物紫巫草（Striga hermonthica）自然种群中宿主特异性相互作用的转录组学
DOI：
10.1017/wsc.2019.20
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发表时间：
2019
期刊：
影响因子：
2.5
通讯作者：
Lasky, Jesse R.
中科院分区：
文献类型：
--
作者：
Lopez, Lua;Bellis, Emily S.;Wafula, Eric;Hearne, Sarah J.;Honaas, Loren;Ralph, Paula E.;Timko, Michael P.;Unachukwu, Nnanna;dePamphilis, Claude W.;Lasky, Jesse R.
寄主特异性相互作用可以维持寄主攻击多种寄主的遗传和表型多样性。寄主多样性反过来可能通过选择遗传分化或对寄主类型的可塑性反应来促进寄生虫多样性。寄生杂草紫独脚金[紫独脚金]。在撒哈拉以南非洲造成了毁灭性的作物损失，并且能够感染广泛的草宿主。尽管有一些证据表明宿主适应和宿主与斯特里加基因型相互作用，但对种内斯特里加基因组多样性知之甚少。本文研究了生长在不同寄主(玉米[Zea mays L.]和谷物高粱[sorghum bicolor (L.)]上的hermonthica种群的转录组多样性。Moench])。我们检测了来自尼日利亚西部各寄主寄生种群地上组织的基因表达变异和等位基因频率的差异。尽管基于宿主的全基因组分化水平较低，但我们确定了一组与每个宿主特异性相关的寄生虫转录本。在宿主与寄生虫相互作用中起重要作用的几种不同功能类别的寄生虫基因在不同宿主上的表达水平和等位基因不同，包括涉及营养转运、防御和发病机制以及植物激素反应的基因。总的来说，我们提供了一组候选转录本，证明了在出现的寄生虫S. hermonthica的营养组织中宿主特异性相互作用。我们的研究显示了寄主特异性过程的信号如何在地面上被检测到，将寄主-寄生虫相互作用的焦点扩展到吸器连接之外。
Host-specific interactions can maintain genetic and phenotypic diversity in parasites that attack multiple host species. Host diversity, in turn, may promote parasite diversity by selection for genetic divergence or plastic responses to host type. The parasitic weed purple witchweed [Striga hermonthica (Delile) Benth.] causes devastating crop losses in sub-Saharan Africa and is capable of infesting a wide range of grass hosts. Despite some evidence for host adaptation and host-by-Striga genotype interactions, little is known about intraspecific Striga genomic diversity. Here we present a study of transcriptomic diversity in populations of S. hermonthica growing on different hosts (maize [Zea mays L.] vs. grain sorghum [Sorghum bicolor (L.) Moench]). We examined gene expression variation and differences in allelic frequency in expressed genes of aboveground tissues from populations in western Nigeria parasitizing each host. Despite low levels of host-based genome-wide differentiation, we identified a set of parasite transcripts specifically associated with each host. Parasite genes in several different functional categories implicated as important in host–parasite interactions differed in expression level and allele on different hosts, including genes involved in nutrient transport, defense and pathogenesis, and plant hormone response. Overall, we provide a set of candidate transcripts that demonstrate host-specific interactions in vegetative tissues of the emerged parasite S. hermonthica. Our study shows how signals of host-specific processes can be detected aboveground, expanding the focus of host–parasite interactions beyond the haustorial connection.