NTP-entry routes in multi-subunit RNA polymerases
NTP-entry routes in multi-subunit RNA polymerases
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DOI：
10.1016/j.tibs.2005.10.001
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发表时间：
2005-12-01
影响因子：
13.8
通讯作者：
Landick, R
中科院分区：
文献类型：
--
作者：
Landick, R
最近对多亚基RNA聚合酶晶体结构的阐释立即揭示了一个谜：三磷酸核苷酸（NTP）底物是如何到达深埋在酶内的活性位点的？普遍的观点是，ntp通过活性位点和酶表面之间类似的20埃长的二级通道进入。最近，另一种观点被提倡；即，ntp从酶主通道的下游DNA部分进入预结合到DNA模板的活性位点。
The recent elucidation of crystal structures for multisubunit RNA polymerases immediately revealed a mystery: how do nucleotide triphosphate (NTP) substrates reach an active site that is buried deep within the enzyme? The prevailing view is that NTPs enter through an similar to 20 angstrom-long secondary channel between the active site and the enzyme surface. Recently, an alternative view has been advocated; namely, NTPs enter the active site pre-bound to the DNA template from the downstream DNA portion of the main channel of the enzyme.