A NON-ENZYMATIC RNA-POLYMERASE MODEL
A NON-ENZYMATIC RNA-POLYMERASE MODEL
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DOI：
10.1126/science.6186026
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
ORGEL, LE
中科院分区：
文献类型：
--
作者：
INOUE, T;ORGEL, LE
以C(胞苷)为主要成分的多核苷酸模板有助于从活化的单核苷酸衍生物的混合物中合成寡核苷酸。当且仅当其补体存在于模板中时，核苷酸才被结合到低聚产物中。该反应具有高保真度，产物的平均链长为6-10个核苷酸。鸟苷以外的碱基被结合在低聚物或其3‘’末端，但很少在其5‘’末端结合。
Polynucleotide templates containing C (cytidine) as the major component facilitate the synthesis of oligonucleotides from mixtures of the activated mononucleotide derivatives. A nucleotide is incorporated into oligomeric products if and only if its complement is present in the template. The reaction has a high fidelity and produces products with mean chain lengths of 6-10 nucleotides. Bases other than guanosine are incorporated within oligomers or at their 3'' termini, but rarely at their 5'' termini.