Laboratory investigation of an E. coli O157:H7 outbreak associated with swimming in Battle Ground Lake, Vancouver, Washington.
Laboratory investigation of an E. coli O157:H7 outbreak associated with swimming in Battle Ground Lake, Vancouver, Washington.
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对与华盛顿州温哥华 Battle Ground Lake 游泳相关的大肠杆菌 O157:H7 爆发进行的实验室调查。
DOI：
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发表时间：
2002
影响因子：
0.8
通讯作者：
T. Newman
中科院分区：
文献类型：
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作者：
M. Samadpour;J. Stewart;K. Steingart;C. Addy;John Louderback;M. Mcginn;J. Ellington;T. Newman
大肠杆菌O157：H7与一些水传播的疫情有关，但从未从相关环境中恢复。本文报道了1999年8月发生的一起大肠杆菌暴发疫情。大肠杆菌O 157：H7与华盛顿克拉克县马特尔格朗德湖游泳有关。E.大肠杆菌O157：H7分离自鸭粪和两个水样。作者使用脉冲场凝胶电泳比较这些分离株与患者分离株的遗传同源性。所有分离物产生相同的限制性片段模式。此外，使用聚合酶链反应，作者发现患者分离株和环境分离株具有相同的毒力因子（Stx，eaeA和hly）。
Escherichia coli O157:H7 has been associated with a number of waterborne outbreaks, but it has never been recovered from an implicated environment. This paper reports on an August 1999 outbreak of E. coli O157:H7 associated with swimming in Battle Ground Lake in Clark Country, Washington. E. coli O157:H7 was isolated from duck feces, as well as from two water samples. The authors used pulsed-field gel electrophoresis to compare these isolates with patient isolates for genetic homology. All the isolates yielded the same restriction fragment patterns. In addition, using polymerase chain reaction, the authors found patient isolates and environmental isolates to have the same virulence factors (Stx, eaeA, and hly).