3D micro-computed tomography of trabecular and cortical bone architecture with application to a rat model of immobilisation osteoporosis
3D micro-computed tomography of trabecular and cortical bone architecture with application to a rat model of immobilisation osteoporosis
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DOI：
10.1007/bf02347054
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发表时间：
2000-05-01
影响因子：
3.2
通讯作者：
Rügsegger, P
中科院分区：
文献类型：
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作者：
Laib, A;Barou, O;Rügsegger, P
骨量和骨微结构是骨强度的主要决定因素。三维微计算机断层扫描具有以非侵入性方式以非常高的分辨率检查小型实验室动物的完整骨骼的潜力。在所提出的工作中，近端部分的后肢卸载和对照组大鼠的胫骨进行了测量与3D MicroCT，并使用直接评价技术，不需要模型假设的扫描区域的次级海绵进行了评价。为了确定完整的骨状态，对胫骨皮质进行了评价，并通过其厚度进行了表征。结果表明，与建议的解剖符合感兴趣的体积（VOI），高达八倍的体积增加相比，可以评估立方或球形VOI。在尾部悬吊23天后观察到约50%的显著的骨小梁损失。新的评估技术表明，大部分骨丢失是由骨小梁变薄引起的，在较小程度上是由其数量减少引起的。最根本的变化是结构类型：剩余的骨骼比对照组的骨骼更像杆状。皮质骨的减少小于骨小梁，23天后仅为-18%。
Bone mass and microarchitecture are the main determinants of bone strength. Three-dimensional micro-computed tomography has the potential to examine complete bones of small laboratory animals with very high resolution in a non-invasive way. In the presented work, the proximal part of the tibiae of hindlimb unloaded and control rats were measured with 3D MicroCT, and the secondary spongiosa of the scanned region was evaluated using direct evaluation techniques that do not require model assumptions. For determination of the complete bone status, the cortex of the tibiae was evaluated and characterised by its thickness. It is shown that with the proposed anatomically conforming volume of interest (VOI), up to an eight-fold volume increase can be evaluated compared to cubic or spherical VOIs. A pronounced trabecular bone loss of -50 % is seen after 23 days of tail suspension. With the new evaluation techniques, it is shown that most of this bone loss is caused by the thinning of trabeculae, and to a lesser extent by a decrease in their number. What changes most radically is the structure type: the remaining bone is more rod-like than the control group's bone. Cortical bone decreases less than trabecular bone, with only -18 % after 23 days.