Myxococcus cells respond to elastic forces in their substrate
Myxococcus cells respond to elastic forces in their substrate
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DOI：
10.1073/pnas.96.14.8052
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发表时间：
1999-07-06
影响因子：
11.1
通讯作者：
Kaiser, D
中科院分区：
文献类型：
--
作者：
Fontes, M;Kaiser, D
弹性运动描述了黄色粘球菌细胞感受并响应其所停留的琼脂凝胶内的弹性力的能力。在施加应力的5分钟内，每个细胞开始重新定向其长轴垂直于应力。然后细胞向那个方向滑动，群体变得不对称。一个可量化的测定的强度的elasticotaxis是基于变化的群体形状从圆形到椭圆形。通过使用等基因运动突变体的集合，已经发现在琼脂中响应应力的能力完全取决于冒险（A）运动，而根本不取决于社会（S）运动或frz基因。事实上，S-突变体（其仅通过A运动性移动）比野生型更强烈地响应所施加的应力，尽管它们的铺展速率比wt菌株的铺展速率慢。基于群集和elasticotactic表型的同基因卷曲株，也有缺陷的A或S运动，frz的行为，如果S运动系统的一部分。
Elasticotaxis describes the ability of Myxococcus xanthus cells to sense and to respond to elastic forces within an agar gel on which they rest. Within 5 min of the application of stress, each cell begins to reorient its long axis perpendicular to the stress force. The cells then glide in that direction, and the swarm becomes asymmetric. A quantifiable assay for the strength of elasticotaxis is based on the change in swarm shape from circular to elliptic. By using a collection of isogenic motility mutants, it has been found that the ability to respond to stress in agar depends totally on adventurous (A) motility, but not at all on social (S) motility or on the frz genes. In fact, S- mutants (which are moving only by means of A motility) respond to the applied stress more strongly than does the wild type, despite the fact that their spreading rates are slower than that of the wt strain. Based on the swarming and elasticotactic phenotypes of isogenic frizzy strains that were also defective either in A or S motility,frz behaves as if part of the S motility system.