Administration of Pediococcus acidilactici or Saccharomyces cerevisiae boulardii modulates development of porcine mucosal immunity and reduces intestinal bacterial translocation after Escherichia coli challenge
Administration of Pediococcus acidilactici or Saccharomyces cerevisiae boulardii modulates development of porcine mucosal immunity and reduces intestinal bacterial translocation after Escherichia coli challenge
复制标题
DOI：
10.2527/jas.2008-0919
复制
发表时间：
2009-03-01
影响因子：
3.3
通讯作者：
Fairbrother, J. M.
中科院分区：
文献类型：
--
作者：
Lessard, M.;Dupuis, M.;Fairbrother, J. M.
本研究探讨了益生菌乳酸片球菌（PA）和布拉氏酿酒酵母（SCB）对猪肠道免疫特性和抗产肠毒素大肠杆菌（ETEC）感染的影响。在分娩前两周，将30头母猪及其未来的窝仔分配到以下处理组：1）不含抗生素或益生菌处理的对照组（CTRL），2）在断奶饲料中添加抗生素（泰妙菌素）的对照组（ABT），或从出生后24小时起用3）PA、4）SCB或5）PA+SCB处理的窝仔。在哺乳期，PA，SCB，或PA+SCB每周3次灌胃仔猪。在21日龄断奶后，在日粮中添加益生菌或ABT。每窝选择4头猪评价性能和叶酸和维生素B-12的血液浓度。其中3只在第49 - 51天用ETEC菌株经口攻毒，并在第52天处死。其余窝中的3头仔猪在第18天屠宰，另外3头在第24天屠宰。采集血液、回肠和肠系膜淋巴结（MLN）样本，以表征白细胞群，测定回肠冲洗液中的伊加浓度，并评价MLN中的细菌移位。未观察到对断奶后性能以及叶酸和维生素B-12血液浓度的给药影响。在回肠中，用PA处理的18日龄哺乳仔猪中的CD 4（-）CD 8（+low）T细胞的百分比高于CTRL和PA+SCB组（P = 0.05）。在MLN中，CD 8（+）T细胞的百分比在第18天和第24天不受任何处理的影响，但在断奶后下降（P = 0.006）。在血液中，CD 8（+）T细胞不受治疗或断奶的影响。在ETEC攻毒后（第52天），与CTRL相比，PA、SCB、PA+SCB或ABT处理的猪中MLN的细菌移位减少（P = 0.05）。ETEC激发后，未观察到对血液白细胞群的治疗效应，尽管时间效应（第42天与第52天）表明，与第42天相比，第52天血液CD 4（+）和γ δ-T淋巴细胞增加（P < 0.05），而CD 4（-）CD 8（+低）T淋巴细胞和单核细胞显著减少（P < 0.01）。最后，伊加浓度回肠冲洗收集的第42和52天SCB和CTRL仔猪比ABT和PA仔猪。总之，益生菌可能具有调节肠道中淋巴细胞群和伊加分泌的潜力，并减少ETEC感染后细菌移位至MLN。
In this study, the influence of the probiotics, Pediococcus acidilactici (PA) and Saccharomyces cerevisiae boulardii (SCB), on intestinal immune traits and resistance to enterotoxigenic Escherichia coli (ETEC) infection was evaluated in pigs. Two weeks before farrowing, 30 sows and their future litters were allocated to the following treatments: 1) control group without antibiotic or probiotic treatment (CTRL), 2) control with antibiotic (tiamulin) added to weanling feed (ABT), or litters treated with 3) PA, 4) SCB, or 5) PA+SCB from 24 h after birth. During lactation, PA, SCB, or PA+SCB were given to piglets 3 times a week by gavage. After weaning at 21 d of age, probiotics or ABT were added to the diet. Four pigs per litter were chosen to evaluate performance and blood concentrations of folic acid and vitamin B-12. Three of these were orally challenged with an ETEC strain on d 49 to 51 and killed on d 52. Three piglets from the rest of the litter were slaughtered on d 18 and 3 others on d 24. Blood, ileum, and mesenteric lymph node (MLN) samples were taken to characterize leukocyte populations, determine IgA concentrations in ileal flushes, and evaluate bacterial translocation in MLN. No treatment effect on postweaning performance and on blood concentrations of folic acid and vitamin B-12 was observed. In the ileum, the percentage of CD4(-)CD8(+low) T cells was greater (P = 0.05) in 18-d-old nursed piglets treated with PA than in those of the CTRL and PA+SCB groups. In the MLN, the percentage of CD8(+) T cells was not affected by any of the treatments at d 18 and 24 but decreased (P = 0.006) after weaning. In the blood, CD8(+) T cells were not affected by treatments or weaning. After the ETEC challenge (d 52), bacterial translocation to MLN was reduced (P = 0.05) in pigs treated with PA, SCB, PA+SCB, or ABT compared with CTRL. No treatment effect was observed on blood leukocyte populations after ETEC challenge, although a time effect (d 42 vs. 52) indicated that blood CD4(+) and gamma delta-T lymphocytes were increased (P < 0.05) on d 52 compared with d 42, whereas CD4(-)CD8(+low) T lymphocytes and monocytes were markedly reduced (P < 0.01). Finally, the IgA concentration in ileal flushes collected on d 42 and 52 was greater in SCB and CTRL piglets than in ABT and PA piglets. In conclusion, probiotics may have the potential to modulate establishment of lymphocyte populations and IgA secretion in the gut and to reduce bacterial translocation to MLN after ETEC infection.