Biological activities of single-chain goldfish follicle-stimulating hormone and luteinizing hormone
Biological activities of single-chain goldfish follicle-stimulating hormone and luteinizing hormone
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DOI：
10.1016/j.aquaculture.2007.12.002
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发表时间：
2008-02-05
期刊：
影响因子：
4.5
通讯作者：
Kobayashi, Makito
中科院分区：
文献类型：
--
作者：
Hayakawa, Youichi;Morita, Tetsuro;Kobayashi, Makito
重组金鱼促性腺激素（GTHs）、促卵泡激素（FSH）和促黄体生成激素（LH）已被杆状病毒感染家蚕幼虫。这些GTH是由共同的α亚基和FSH β或LH β亚基组成的异二聚体激素; GTH亚基分别在家蚕幼虫中产生。为了简化激素的生产过程并提高生产效率，我们生产了单链FSH（scFSH）和LH（scLH），其中FSH β或LH β亚基与α亚基融合。利用杆状病毒-家蚕幼虫系统生产的单链GTH对雄性金鱼、雌性红点？和雄性日本鳗鲡进行了体内生物学活性分析。注射含有scFSH或scLH的血淋巴诱导雄性金鱼产生精液。scLH诱导排卵，但scFSH表现出较低的效力。scLH和scFSH在以7天间隔5-6次施用重组GTH后诱导性未成熟雄性鳗鱼睾丸中精子发生的启动。在施用scLH的鳗鱼中观察到精子，在施用scFSH的鳗鱼中观察到精子细胞，而施用未处理的家蚕幼虫的血淋巴的鳗鱼的睾丸保持在精原细胞阶段。因此，由杆状病毒在家蚕幼虫中产生的金鱼单链GTH不仅在配子发生的最后阶段（产生精液和排卵）而且在配子发生的早期阶段（精子发生）显示出体内生物学效力。我们认为，单链鱼GTH可以用于诱导性腺发育的水产养殖鱼类。(c)2007 Elsevier B.V保留所有权利。
Recombinant gonadotropins (GTHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) of goldfish Carassius auratus were previously produced by baculovirus in silkworm larvae Bombyx mori. These GTHs are heterodimeric hormones consisting of a common a subunit and an FSH beta or LH beta subunit; the GTH subunits were produced separately in silkworm larvae. In order to simplify the production process of the hormones and increase production efficiency, we produced single-chain FSH (scFSH) and LH (scLH) in which the FSH beta or LH beta subunit was fused to the a subunit. Biological activities of these single-chain GTHs produced by baculovirus-silkworm larvae system were analyzed in vivo using male goldfish, female bitterling Rhodeus ocellatus ocellatus, and male Japanese eel Anguilla japonica. Injection of hemolymph containing scFSH or scLH induced milt production in male goldfish. scLH induced ovulation in bitterling but scFSH showed lower potency. Both scLH and scFSH induced the initiation of spermatogenesis in the testes of sexually immature male eels after 5-6 administrations of the recombinant GTHs at seven day intervals. Spermatozoa were observed in scLH-administered eels and spermatids in scFSH-administered eels whereas the testes of eels administered with hemolymph of untreated silkworm larvae remained at the spermatogonia stage. Thus, single-chain GTHs of goldfish produced by baculovirus in silkworm larvae showed in vivo biological potency not only in the final phase of gametogenesis (milt production and ovulation) but also in the early phase of gametogenesis (spermatogenesis). We suggest that single-chain fish GTHs could be used for the induction of gonadal development in aquaculture fishes. (c) 2007 Elsevier B.V All rights reserved.