Production of beta-defensins by human airway epithelia.
Production of beta-defensins by human airway epithelia.
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DOI：
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发表时间：
1998
影响因子：
11.1
通讯作者：
P. K. Singh;H. Jia;K. Wiles;J. Hesselberth;L. Liu;B. Conway;E. Greenberg;E. Valore;M. Welsh
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文献类型：
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作者：
P. K. Singh;H. Jia;K. Wiles;J. Hesselberth;L. Liu;B. Conway;E. Greenberg;E. Valore;M. Welsh
人β-防御素（HBDs）是一种抗菌肽，可能在粘膜防御中发挥作用。这些肽的活性降低与囊性纤维化（CF）肺病的发病机制有关。我们发现HBD-1和HBD-2 mRNA在非CF和CF患者切除的表面和粘膜下腺上皮中表达。促炎细胞因子白细胞介素-1 β刺激原代培养的气道上皮细胞中HBD-2的表达，但不刺激HBD-1 mRNA和肽的表达。HBD-1在正常志愿者、CF患者和炎性肺病患者的支气管肺泡灌洗液（BAL）中被发现，而HBD-2在CF或炎性肺病患者的BAL液中被检测到，但在正常志愿者中未检测到。HBD-1和HBD-2在BAL液中的浓度均为几ng/ml，两种重组肽均显示盐敏感性杀菌活性。这些数据表明，在肺中，HBD-2的表达是由炎症诱导的，而HBD-1可能在没有炎症的情况下起防御作用。
Human beta-defensins (HBDs) are antimicrobial peptides that may play a role in mucosal defense. Diminished activity of these peptides has been implicated in the pathogenesis of cystic fibrosis (CF) lung disease. We show that HBD-1 and HBD-2 mRNAs are expressed in excised surface and submucosal gland epithelia from non-CF and CF patients. The pro-inflammatory cytokine interleukin-1beta stimulated the expression of HBD-2 but not HBD-1 mRNA and peptide in primary cultures of airway epithelia. HBD-1 was found in bronchoalveolar lavage (BAL) fluid from normal volunteers, CF patients, and patients with inflammatory lung diseases, whereas HBD-2 was detected in BAL fluid from patients with CF or inflammatory lung diseases, but not in normal volunteers. Both HBD-1 and HBD-2 were found in BAL fluid in concentrations of several ng/ml, and both recombinant peptides showed salt-sensitive bactericidal activity. These data suggest that in the lung HBD-2 expression is induced by inflammation, whereas HBD-1 may serve as a defense in the absence of inflammation.