SEROTYPING OF EUROPEAN ISOLATES OF CHLAMYDIA-PSITTACI FROM POULTRY AND OTHER BIRDS
SEROTYPING OF EUROPEAN ISOLATES OF CHLAMYDIA-PSITTACI FROM POULTRY AND OTHER BIRDS
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DOI：
10.1128/jcm.31.1.134-137.1993
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发表时间：
1993-01-01
影响因子：
9.4
通讯作者：
HAESEBROUCK, F
中科院分区：
文献类型：
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作者：
VANROMPAY, D;ANDERSEN, AA;HAESEBROUCK, F
一组五血清型特异性单克隆抗体，区分五个已知的禽血清型鹦鹉热衣原体被用来血清型45欧洲禽鹦鹉热衣原体分离株。衣原体抗原在布法罗绿色猴（BGM）细胞或鸡胚中生长，然后接种到BGM细胞中。在间接免疫荧光试验中进行血清分型。45个欧洲分离物包括22个来自鹦鹉形目的分离物、9个来自鸽形目的分离物、6个来自鸡形目的分离物、5个来自雀形目的分离物和3个来自雁形目的分离物。所有这些都成功地进行了血清分型。一个来自鸭的分离株和两个来自鹦鹉的分离株用两种被认为对两种不同血清型具有特异性的单克隆抗体给出阳性免疫荧光。通过琼脂覆盖法克隆了这三个分离物。克隆的血清分型表明，鸭和鹦鹉各感染了两种不同的血清型。克隆后，一个来自鹦鹉的分离株仅与血清型A反应。从这些结果可以得出结论，该血清分型系统允许对迄今为止检测的所有分离株进行分类。结果表明，类似的血清型在欧洲和美国的鸟类中普遍存在。结果还表明，来自某一目的鸟类更容易感染不同的血清型。在免疫荧光试验中使用一组血清型特异性单克隆抗体，为禽类分离株的血清分型提供了可靠的方法。新的禽分离血清型的单克隆抗体可以很容易地添加到面板，这使得该系统用于流行病学研究。
A panel of five serovar-specific monoclonal antibodies which distinguish the five known avian serovars of Chlamydia psittaci was used to serotype 45 European avian Chlamydia psittaci isolates. Chlamydial antigen was grown in Buffalo green monkey (BGM) cells or in embryonated chicken eggs and was then inoculated into BGM cells. Serotyping was performed in an indirect immunofluorescence test. The 45 European isolates included 22 isolates from the order Psittaciformes, 9 isolates from the order Columbiformes, 6 isolates from the order Galliformes, 5 isolates from the order Passeriformes, and 3 isolates from the order Anseriformes. All of these were successfully serotyped. No additional serovars were found. One isolate from a duck and two isolates from psittacine birds gave positive immunofluorescences with two monoclonal antibodies considered to be specific for two different serovars. These three isolates were cloned by an agar overlay method. Serotyping of the clones demonstrated that the duck and one psittacine bird each were infected with two different serovars. After cloning, one isolate from a psittacine bird reacted only with serovar A. From these results it was concluded that this serotyping system allows the classification of all isolates tested so far. The results show that similar serovars are prevalent in avian species in Europe and the United States. The results also indicate that birds from a certain order are more susceptible to a distinct serovar. The use of a panel of serovar-specific monoclonal antibodies in the immunofluorescence test provides a reliable method for serotyping avian isolates. Monoclonal antibodies to new avian isolate serovars can easily be added to the panel, which makes the system useful for epidemiological studies.