Maternal control of seed oil content in Brassica napus: the role of silique wall photosynthesis
Maternal control of seed oil content in Brassica napus: the role of silique wall photosynthesis
复制标题
甘蓝型油菜种子含油量的母体控制：长角果壁光合作用的作用
DOI：
10.1111/j.1365-313x.2011.04802.x
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发表时间：
2012-02-01
期刊：
影响因子：
7.2
通讯作者：
Wang, Han-Zhong
中科院分区：
文献类型：
--
作者：
Hua, Wei;Li, Rong-Jun;Wang, Han-Zhong
种子含油量是油菜的重要农艺性状。然而，我们对控制石油聚集的调节过程的理解仍然有限。以油分含量不同的两个油菜品系（zy 036和51070）为材料，研究发现母本基因型对种子油分含量有很大影响。遗传和生理证据表明，角果壁（母体组织）的局部和组织特异性光合活性的差异是导致种子油含量不同的原因。这种效果是模仿植物操纵角果壁光合作用。此外，淀粉含量和重要的脂质合成调控基因WRINKLED 1在发育种子中的表达与角果壁光合活性有关。利用454焦磷酸测序技术研究了zy 036和51070角果壁光合作用差异的可能分子机制。有趣的是，结果表明，光合作用相关的基因在两个总角果壁表达基因和基因型之间的差异表达的基因中均过量表达。一个潜在的调节机制，提高光合作用的zy 036角果壁的基础上，从拟南芥的知识提出。差异表达的核酮糖-1，5-二磷酸羧化酶/加氧酶（Rubisco）相关基因用于进一步研究。油含量与角果壁中BnRBCS 1A表达水平和Rubisco活性密切相关，但与叶片中的油含量无关。两者合计，我们的研究结果突出了角果壁光合作用的重要作用，在种子油含量的调节方面的母亲的影响。
Seed oil content is an important agronomic trait in rapeseed. However, our understanding of the regulatory processes controlling oil accumulation is still limited. Using two rapeseed lines (zy036 and 51070) with contrasting oil content, we found that maternal genotype greatly affects seed oil content. Genetic and physiological evidence indicated that difference in the local and tissue-specific photosynthetic activity in the silique wall (a maternal tissue) was responsible for the different seed oil contents. This effect was mimicked by in planta manipulation of silique wall photosynthesis. Furthermore, the starch content and expression of the important lipid synthesis regulatory gene WRINKLED1 in developing seeds were linked with silique wall photosynthetic activity. 454 pyrosequencing was performed to explore the possible molecular mechanism for the difference in silique wall photosynthesis between zy036 and 51070. Interestingly, the results suggested that photosynthesis-related genes were over-represented in both total silique wall expressed genes and genes that were differentially expressed between genotypes. A potential regulatory mechanism for elevated photosynthesis in the zy036 silique wall is proposed on the basis of knowledge from Arabidopsis. Differentially expressed ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco)-related genes were used for further investigations. Oil content correlated closely with BnRBCS1A expression levels and Rubisco activities in the silique wall, but not in the leaf. Taken together, our results highlight an important role of silique wall photosynthesis in the regulation of seed oil content in terms of maternal effects.