Quantitative analysis of bioactive components in walnut leaves by UHPLC-Q-Orbitrap HRMS combined with QAMS
Quantitative analysis of bioactive components in walnut leaves by UHPLC-Q-Orbitrap HRMS combined with QAMS
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DOI：
10.1016/j.foodchem.2020.127180
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发表时间：
2020-11-30
期刊：
影响因子：
8.8
通讯作者：
Liu, Rongxia
中科院分区：
文献类型：
--
作者：
Su, Chaonan;Li, Caihong;Liu, Rongxia
核桃叶富含酚类成分，具有抗菌和抗氧化作用。然而，对核桃叶中有效成分的定量分析研究较少。本研究将超高效液相色谱-四极杆-轨道高分辨质谱仪(UHPLC-Q-Orbitrap HRMS)与多组分的单标记物定量分析(QAMS)相结合，建立了核桃叶中有效成分的定量分析方法。总共有13种生物活性成分通过一个单一的标记--槲皮素进行了分析。为了评价该方法的准确性，建立了具有13个参考标准的辅助定量方法，并对其进行了验证。定量分析结果的标准方法偏差(SMDS)小于20%，说明QAMS方法能准确测定核桃叶中的有效成分。该方法可为解决其他食品和草药分析缺乏参考标准提供参考。
Walnut leaves are rich in phenolic components with antibiotic and antioxidative effects. However, few studies have reported the quantitative analysis of active components in walnut leaf. In this study, a novel method for quantifying the active components in walnut leaves was developed by combining ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) with quantitative analysis of multi-components by a single marker (QAMS). In total, 13 bioactive components were analyzed by a single marker, quercetin. To evaluate the accuracy of this method, an auxiliary quantification method with 13 reference standards was established and validated. The standard method differences (SMDs) of the quantification results between QAMS and the auxiliary method were lower than 20%, indicating that the QAMS method can accurately determine the active components in walnut leaves. This method can provide a reference to address the absence of reference standards for analyzing other foods and herbs.