Cytogenetic and Sequence Analyses of Mitochondrial DNA Insertions in Nuclear Chromosomes of Maize.
Cytogenetic and Sequence Analyses of Mitochondrial DNA Insertions in Nuclear Chromosomes of Maize.
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DOI：
10.1534/g3.115.020677
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发表时间：
2015-09-01
期刊：
影响因子：
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通讯作者：
Newton KJ
中科院分区：
文献类型：
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作者：
Lough AN;Faries KM;Koo DH;Hussain A;Roark LM;Langewisch TL;Backes T;Kremling KA;Jiang J;Birchler JA;Newton KJ
线粒体 DNA (mtDNA) 转移至核基因组是一个经常发生的过程，已在许多物种中观察到。然而，很少有研究关注物种内核线粒体 DNA 序列 (NUMT) 的变异。这项研究检查了不同玉米亚种染色体中的 mtDNA 插入。通过使用荧光原位杂交获得mays（玉米）自交系。在四个自交系（B73、M825、HP301 和 Oh7B）中，在 9 号染色体长臂 (9L) 的大致相同位置上鉴定出相对较大的 NUMT。对 B73 和 M825 两个品系中类似位置的 9L NUMT 的进一步检查表明，这些位点的大尺寸是由于大多数线粒体基因组的存在；然而，在公开的 B73 9 号染色体核序列中仅发现了该 NUMT 的一部分（总计约 252 kb）。纤维荧光原位杂交分析估计 B73 9L NUMT 的大小为约 1.8 Mb，并表明 NUMT 已甲基化。 9L NUMT 内的两个 mtDNA 区域（2.4 kb 和 3.3 kb）不存在于 B73 线粒体 NB 基因组中；然而，这些 2.4-kb 和 3.3-kb 片段也存在于其他玉米线粒体基因组中，包括玉米亚种 (Zea mays ssp)。 parviglumis，驯化玉米的祖先。
The transfer of mitochondrial DNA (mtDNA) into nuclear genomes is a regularly occurring process that has been observed in many species. Few studies, however, have focused on the variation of nuclear-mtDNA sequences (NUMTs) within a species. This study examined mtDNA insertions within chromosomes of a diverse set of Zea mays ssp. mays (maize) inbred lines by the use of fluorescence in situ hybridization. A relatively large NUMT on the long arm of chromosome 9 (9L) was identified at approximately the same position in four inbred lines (B73, M825, HP301, and Oh7B). Further examination of the similarly positioned 9L NUMT in two lines, B73 and M825, indicated that the large size of these sites is due to the presence of a majority of the mitochondrial genome; however, only portions of this NUMT (∼252 kb total) were found in the publically available B73 nuclear sequence for chromosome 9. Fiber-fluorescence in situ hybridization analysis estimated the size of the B73 9L NUMT to be ∼1.8 Mb and revealed that the NUMT is methylated. Two regions of mtDNA (2.4 kb and 3.3 kb) within the 9L NUMT are not present in the B73 mitochondrial NB genome; however, these 2.4-kb and 3.3-kb segments are present in other Zea mitochondrial genomes, including that of Zea mays ssp. parviglumis, a progenitor of domesticated maize.