Cofactor induced synthesis of D-glucose-6-phosphate: NADP oxidoreductase in the uterus.
Cofactor induced synthesis of D-glucose-6-phosphate: NADP oxidoreductase in the uterus.
复制标题
辅因子诱导 D-葡萄糖-6-磷酸合成：子宫内的 NADP 氧化还原酶。
作者：
K. Barker
去卵巢大鼠子宫中d -葡萄糖-6-磷酸：NADP氧化还原酶（G6PD）的合成被证明受细胞内NADP浓度的控制。采用非手术方法将5.0 μmol NADP直接注入子宫腔内。这种治疗增加子宫总G6PD活性的程度与单次注射（iv） 5.0 μg雌二醇-17β在12小时内相同。剂量响应曲线显示，当NADP剂量达到5.0 μmol /只时，子宫G6PD活性呈线性增加。对NADP反应的动力学表现为2小时滞后期，随后G6PD活性增加，持续24小时。NADPH与NADP在诱导G6PD合成方面同样有效。与NADP相比，NAD和NADH对G6PD活性的促进作用要小得多，而非那嗪甲硫代硫酸钠、组胺和葡萄糖的腔内应用没有效果。放线菌素D和环己亚胺在腹腔内应用的剂量响应。
The synthesis of D-glucose-6- phosphate: NADP oxidoreductase (G6PD) in the uterus of the ovariectomized rat has been shown to be controlled by the intracellular concentrations of NADP. A nonsurgical method was used to apply 5.0 μmoles of NADP directly into the lumen of the uterus. This treatment increased the total uterine G6PD activity to the same extent as a single injection (iv) of 5.0 μg of estradiol-17β over a 12 hr period. A dose response curve indicated linear increases in uterine G6PD activity up to the dose of 5.0 μmoles of NADP/ animal. The kinetics of the response to NADP show a 2 hr lag phase followed by increased G6PD activity through the 24th hr. NADPH was equally as effective as NADP in the induction of G6PD synthesis. NAD and NADH promoted increased G6PD activity to a much lesser degree than NADP, and intraluminal application of phenazine methosulfate, histamine and glucose was without effect. A dose responsecurve of the ability of actinomycin D and cycloheximide, applied intraluminally, t...