Diagnostic value of serum EBV-DNA quantification and antibody to viral capsid antigen in nasopharyngeal carcinoma patients
Diagnostic value of serum EBV-DNA quantification and antibody to viral capsid antigen in nasopharyngeal carcinoma patients
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DOI：
10.1111/j.1349-7006.2004.tb03241.x
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发表时间：
2004-06-01
期刊：
影响因子：
5.7
通讯作者：
Yoshizaki, T
中科院分区：
文献类型：
--
作者：
Kondo, S;Horikawa, T;Yoshizaki, T
采用实时荧光定量聚合酶链式反应技术，对以台湾为代表的鼻咽癌高发区和以日本为代表的低发区鼻咽癌患者血清EB病毒DNA(EBV-DNA)的检测进行了比较。41例日本鼻咽癌患者和23例台湾地区鼻咽癌患者血清EBV-DNA中位数分别为5450拷贝/ml和2125拷贝/ml，鼻咽癌患者血清EBV-DNA中位数均显著高于对照组。用受试者工作特征曲线测定EB病毒DNA定量的敏感性和特异性(临界点为6.87拷贝/ml；敏感性为0.855；特异性为0.885)，并与EB病毒衣壳抗原滴度进行比较，结果表明EB病毒DNA是一项比EB病毒衣壳抗原滴度更敏感和特异的指标。然后，我们分析了19例复发的鼻咽癌患者(11例日本人和8例台湾人)和26例持续缓解的鼻咽癌患者。虽然日本患者和台湾患者的EBV-DNA值无显著差异，但19例复发患者的EBV-DNA值显著高于缓解者。ROC分析再次显示EBV-DNA比EBV-VCA具有更高的诊断价值。这些结果表明，在鼻咽癌高发区和低发区，EBV-DNA是比EBV-VCA更可靠的肿瘤标志物。
We compared the amount of serum Epstein-Barr virus DNA (EBV-DNA) detected in patients with nasopharyngeal carcinoma (NPC) in a high-incidence area, represented by Taiwan, and a low-incidence area, represented by Japan, using real-time quantitative PCR. The median serum EBV-DNA value in 41 Japanese NPC cases was 5450 copies/ml, and that in in 23 Taiwanese cases was 2125 copies/ml. The median serum EBV-DNA value in all 64 NPC cases was significantly higher than in control groups. Using receiver-operating-characteristic (ROC) curves, the sensitivity and specificity of EBV-DNA quantification were determined (cut-off point, 6.87 copies/ml; sensitivity, 0.855; specificity, 0.885) and compared with those of EBV-viral-capsid-antigen (VCA) titers; the results showed that EBV-DNA was a more sensitive and specific parameter than EBV-VCA titer. Then, we analyzed 19 NPC patients in whom recurrence developed (11 Japanese and 8 Taiwanese), and 26 NPC patients in continuous remission. Although there was no significant difference in EBV-DNA values between Japanese and Taiwanese patients, the value was significantly higher in the 19 patients with recurrence than in those in remission. ROC analysis again revealed a higher diagnostic value of EBV-DNA than EBV-VCA. These results suggest EBV-DNA is a more reliable tumor marker than EBV-VCA in both high-incidence and low-incidence areas of NPC.