Proboscis extension response (PER) assay in Drosophila.
Proboscis extension response (PER) assay in Drosophila.
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DOI：
10.3791/193
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Carlson, John R
中科院分区：
文献类型：
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作者：
Shiraiwa, Takashi;Carlson, John R
喙延伸反应（PER）是一种味觉行为测定，已在苍蝇和蜜蜂中使用。在苍蝇嘴巴的表面（唇瓣）上，有毛发状的结构，叫做感受器，里面有味觉神经元。当有吸引力的物质接触到唇瓣时，苍蝇就会伸出它的喙去消耗这种物质。喙延伸反应（PER）测定了这种味觉行为反应，是了解单个果蝇食物偏好的有效方法。各种糖的溶液，如蔗糖、葡萄糖和果糖，对苍蝇非常有吸引力。当在甜溶液中混合时，厌恶物质的作用也可以作为PER的降低来测试。尽管基本程序很简单，但有许多事情会阻碍它的工作。需要注意的因素之一是苍蝇的反应状态。使果蝇达到适当反应状态所需的饥饿时间从36小时到72小时不等。我们建立了一系列的控制来评估苍蝇的状态，并允许筛选出无反应或高反应的个体动物。另一个重要的因素是撞击程度和接触唇瓣的位置，这很难用语言来描述。本视频演示演示了所有这些以及其他一些改进，这些改进将增加该方法的可重复性。
Proboscis extension response (PER) is a taste behavior assay that has been used in flies as well as in honeybees.On the surface of the fly's mouth (labellum), there are hair-like structures called sensilla which houses taste neurons. When an attractive substance makes contact to the labellum, the fly extends its proboscis to consume the material. Proboscis Extension Response (PER) assay measures this taste behavior response, and it is a useful method to learn about food preferences in a single fly. Solutions of various sugars, such as sucrose, glucose and fructose, are very attractive to the fly. The effect of aversive substances can also be tested as reduction of PER when mixed in a sweet solution.Despite the simplicity of the basic procedure, there are many things that can prevent it from working. One of the factors that requires attention is the fly's responsive state. The required starvation time to bring the fly to the proper responsive state varies drastically from 36 to 72 hours. We established a series of controls to evaluate the fly's state and which allows screening out of non-responsive or hyper-responsive individual animals. Another important factor is the impact level and the position of the contact to the labellum, which would be difficult to describe by words. This video presentation demonstrates all these together with several other improvements that would increase the reproducibility of this method.