Lactoferrin Levels in Human Milk after Preterm and Term Delivery
Lactoferrin Levels in Human Milk after Preterm and Term Delivery
复制标题
DOI：
10.1055/s-0036-1586105
复制
发表时间：
2016-09-01
影响因子：
2
通讯作者：
Magaldi, Rosario
中科院分区：
文献类型：
--
作者：
Albenzio, Marzia;Santillo, Antonella;Magaldi, Rosario
背景乳铁蛋白（LF）是母乳中一种高度代表性的功能性糖蛋白，在新生儿中发挥广泛的抗感染、免疫调节和益生元作用。有限的数据是可用的评估浓度和水平的LF在母乳中随着时间的推移，在哺乳期的母亲谁交付婴儿在不同的GA。我们的目的与本研究是确定LF的水平在母乳中的早产儿和足月儿的母亲，并评估在不同的时间从交付的变化，在初乳和成熟milk.Methods早产儿和足月儿的母亲在福贾，意大利，新生儿病房，接近并参加了这项研究。从每个母亲，在出生后的前3天内（A组，0-72小时），在分娩后的第5和第7天之间（B组，120-168小时），和在第10天之后（C组，> 240小时）收集乳汁样品。根据婴儿的GA将所有乳汁样品分为五组：GA 24至27.6周（I），GA 28至31.6周（II），GA 32至34.6周（III），GA 35至37.6周（IV）和GA> 38周（V）。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法测定乳果糖含量。我们发现，婴儿的GA，以及采样的时间，影响乳中LF的水平。一方面，与其他组相比，I组（GA：24-27.6周）人乳中的LF显示出更高的含量（p < 0.01），并且初乳中的LF水平与GA显著相关（r = 0.31; p < 0.05）。另一方面，乳中LF含量随着时间的推移有明显的下降趋势。总体而言，婴儿出生体重低于1，400 g的早产儿母乳中检测到的LF值最高。将约350 μ g/mL确定为生理平均值。LF含量在我们的population.Conclusions人乳中的LF水平随着时间的推移在哺乳期和根据GA显着变化。人乳中LF含量的这种变化可能反映了不同婴儿在生命早期和几周内的不同需求。这些数据可能有助于为模型提供信息，以设计在托儿所和家庭出院后量身定制的LF补充策略。
Background Lactoferrin (LF) is a highly represented, functional glycoprotein in human milk, exerting a wide range of anti-infective, immunomodulatory, and prebiotic actions in the neonate. Limited data are available assessing the concentrations and levels of LF in maternal milk over time during lactation in mothers who delivered infants at different GAs. Our aim with the present study was to determine the levels of LF in human milk from mothers of preterm and term infants and to evaluate the variations at a different time from delivery, in colostrum and mature milk.Methods Mothers of preterm and term infants from the Neonatology Unit in Foggia, Italy, were approached and enrolled in this study. From each mother, milk samples were collected within the first 3 days after birth (group A, 0-72 hours), between the 5th and 7th day after delivery (group B, 120-168 hours), and after the 10th day (group C, > 240 hours). All milk samples were divided into five groups, according to the GA of the infants: 24 to 27.6 weeks of GA (I), 28 to 31.6 weeks of GA (II), 32 to 34.6 weeks of GA (III), 35 to 37.6 weeks of GA (IV), and > 38 weeks of GA (V). Milk samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the content of LF.Results A total of 84 milk samples were collected from 28 mothers. We found that infant's GA, as well as the time of sampling, affected the levels of LF in milk. On one hand, LF showed higher content in human milk from group I (GA: 24-27.6 weeks) compared with the other groups (p < 0.01), and the levels of LF in colostrum were significantly correlated with GA (r = 0.31; p < 0.05). On the other hand, the LF content of milk had a significant decreasing trend over time. Overall, the highest values of LF were detected in preterm infants' maternal milk with a baby birth weight, lower than 1,400 g. Approximately 350 mu g/mL was identified as the mean, physiological. LF content in human mature milk in our population.Conclusions Levels of LF in human milk vary significantly over time during lactation and according to GA. This variability in the LF content of human milk may reflect the different needs of different infants during the early days and weeks of life. These data might help to inform models to design tailored supplementation strategies of LF in the nurseries and after home discharge.