Measurement of meningeal blood vessel diameter in vivo with a plug-in for ImageJ
Measurement of meningeal blood vessel diameter in vivo with a plug-in for ImageJ
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DOI：
10.1016/j.mvr.2010.04.004
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发表时间：
2010-09-01
影响因子：
3.1
通讯作者：
Messlinger, Karl
中科院分区：
文献类型：
--
作者：
Fischer, Michael J. M.;Uchida, Sae;Messlinger, Karl
血管直径的变化可以手动测量，但这是耗时的，往往不切实际。对于自动测量，商业解决方案是可用的，但专有算法及其潜在缺点不为用户所知。我们提出了一种方法，结合免费和开源的ImageJ软件进行离线分析的CCD相机的图像采集。减影图像允许评估整个视场中的直径变化。编写了半高全宽算法插件以测量血管直径。对于跨血管的给定线，整个图像堆栈中具有平行移位的五次测量的结果被复制到剪贴板。为了验证该方法，使用了已建立的体内模型，即大鼠硬脑膜中的血管变化，反映了传入神经元的活性。静脉注射肾上腺素能受体拮抗剂酚妥拉明可抑制局部电刺激硬脑膜引起的脑膜小动脉血管收缩，而CGRP受体拮抗剂olcegepant则可增强血管收缩。所描述的方法允许用户快速评估在任何选定位置处的整个采集场中的血管直径变化。(C)2010年爱思唯尔公司All rights reserved.
Changes in blood vessel diameter can be measured manually, but this is time-consuming and often impractical. For automatic measurement commercial solutions are available, but the proprietary algorithms and their potential shortcomings are not known to the user. We present an approach with a CCD camera for image acquisition combined with free and open source ImageJ software for offline analysis. A subtraction image allows for the evaluation of the diameter changes throughout the field of view. A full width at half-maximum algorithm plug-in was written to measure the vessel diameter. For a given line across a vessel, the results of five measurements with parallel shifts throughout an image stack are copied to the clipboard. For validation of this method an established in vivo model was used, namely vascular changes in the rat dura mater, reflecting the activity of the afferent neurons. Vasoconstriction of the meningeal arterioles induced by local electrical stimulation of the dura was inhibited by intravenous administration of the adrenoceptor antagonist phentolamine and amplified by the CGRP receptor antagonist olcegepant. The described methods allow the user to quickly evaluate vessel diameter changes in the whole acquired field at any selected position. (C) 2010 Elsevier Inc. All rights reserved.