Cellular expression of src-suppressed C kinase substrate (SSeCKS) in the peripheral nervous system and sensory organs
Cellular expression of src-suppressed C kinase substrate (SSeCKS) in the peripheral nervous system and sensory organs
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DOI：
10.2220/biomedres.25.155
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发表时间：
2004
影响因子：
1.2
通讯作者：
Tilladit Rung-ruangkijkrai;D. Fujikura;Y. Kon;T. Iwanaga
中科院分区：
文献类型：
--
作者：
Tilladit Rung-ruangkijkrai;D. Fujikura;Y. Kon;T. Iwanaga
Src抑制的C激酶底物（SSeCKS）在多种细胞中表达，通过肌动蛋白骨架的重塑在细胞分化、迁移和塑形中发挥重要作用。本研究采用原位杂交和免疫组织化学方法研究了SSeCKS在小鼠外周神经系统和感觉器官中的细胞分布。在背根神经节和三叉神经节，小型和中型神经元表达SSeCKS，而大型神经元呈阴性或弱阳性反应。在感觉神经节中，SSeCKS mRNA和蛋白在一些卫星细胞中也有明确的阳性信号，这些卫星细胞常被不表达SSeCKS的神经节细胞包裹。在自主神经节（腹腔神经节，盆神经节，肠肌间神经丛），SSeCKS免疫反应仅限于卫星细胞，并没有检测到神经元胞体。在大多数感觉器官中，包括视网膜、Corti器、味蕾和嗅粘膜，SSeCKS均未出现显著的免疫反应。与此相反，犁鼻器显示强烈的和选择性的表达SSeCKS的感觉上皮，只有支持细胞进行免疫标记。SSeCKS免疫反应阳性的雪旺细胞广泛分布于犁鼻神经、嗅神经和背根等神经束中。这些结果表明，SSeCKS可能参与周围神经系统和犁鼻器中的感觉器官的调节和稳态。
Src-suppressed C kinase substrate (SSeCKS) is expressed in a variety of cells and plays an important role in cell differentiation, migration and shaping via remodeling of the actin-based cytoskeleton. In the present study, the cellular distribution of SSeCKS was studied in the peripheral nervous system and sensory organs of mice by in situ hybridization and immunohistochemistry. In the dorsal root ganglia and trigeminal ganglia, small- and medium-sized neurons expressed SSeCKS, whereas large-sized neurons showed negative or faintly positive reactions. Definite positive signals of SSeCKS mRNA and protein in the sensory ganglia were also found in some satellite cells which frequently enveloped ganglion cells without SSeCKS expression. In autonomic ganglia (celiac ganglia, pelvic ganglia, and intestinal myenteric nerve plexus), SSeCKS immunoreactivity was confined to satellite cells, and not detectable in neuronal somata. No significant immunoreactivity for SSeCKS appeared in most sensory organs, including the retina, organ of Corti, taste buds, and olfactory mucosa. In contrast, the vomeronasal organ displayed intense and selective expression of SSeCKS in the sensory epithelium, where only supporting cells were immunolabeled. SSeCKS-immunoreactive Schwann cells were widely distributed in nerve bundles including the vomeronasal nerve, olfactory nerve, and dorsal root. These findings suggest that SSeCKS might be involved in regulation and homeostasis of the peripheral nervous system and of the vomeronasal organ among sensory organs.