Exogenous phytosulfokine α alleviates chilling injury of loquat fruit via regulating sugar, proline, polyamine and γ-aminobutyric acid metabolisms.
Exogenous phytosulfokine α alleviates chilling injury of loquat fruit via regulating sugar, proline, polyamine and γ-aminobutyric acid metabolisms.
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DOI：
10.1016/j.foodchem.2023.137729
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发表时间：
2023-10
期刊：
影响因子：
8.8
通讯作者：
Yu Liu;Y. Hou;Binghan Yi;Yaqing Zhao;Yinqiu Bao;Zhengguo Wu;Yong Zheng;P. Jin
中科院分区：
文献类型：
--
作者：
Yu Liu;Y. Hou;Binghan Yi;Yaqing Zhao;Yinqiu Bao;Zhengguo Wu;Yong Zheng;P. Jin
采后枇杷果实在低温胁迫下易受冷害。以枇杷为试材，研究了植物磺因子α（phytosulfokine α，PSKα）对冷藏期间糖、脯氨酸、多胺和γ-氨基丁酸（gamma-aminobutyric acid，GABA）代谢的影响。结果表明，PSKα处理显著提高了枇杷果实中PSKα含量沿着上调了EjPSK 3和EjPSK 6的表达，抑制了枇杷果实内部布朗宁指数、电解质外渗率和丙二醛（MDA）含量的增加。此外，PSKα处理还通过激活生物合成途径和抑制CAT途径维持了枇杷果实中还原糖、脯氨酸、多胺和GABA含量的较高水平。相关分析结果表明，PSKα含量与还原糖、脯氨酸、多胺和GABA含量呈正相关。这些结果表明，经PSKα处理后的枇杷果实耐冷性的提高是由于还原糖、脯氨酸、多胺和GABA含量的增加，而这些含量可能受到内源性PSKα信号的调节。
Postharvest loquat fruit is susceptible to chilling injury (CI) under cold stress. In this study, the effects of phytosulfokine α (PSKα) on sugar, proline, polyamine and γ-aminobutyric acid (GABA) metabolisms in loquat fruit during cold storage were investigated. The results showed that PSKα treatment significantly increased PSKα content along with up-regulatingEjPSK3andEjPSK6expressions, and inhibited the increases of internal browning index, electrolyte leakage and malondialdehyde (MDA) content of loquat fruit. Besides, PSKα treatment maintained higher reducing sugar, proline, polyamines, and GABA contents in loquat fruit via activating biosynthesis pathway and suppressing catabolism pathway. More importantly, the results of correlation analysis indicated that PSKα content displayed positive correlations with reducing sugar, proline, polyamines and GABA contents. These findings suggested that the improved chilling tolerance in PSKα-treated loquat fruit was due to enhancing reducing sugar, proline, polyamines, and GABA contents, which might be modulated by endogenous PSKα signaling.