Aging-related changes in the sensitivity of behavioral effects of the neonicotinoid pesticide clothianidin in male mice
Aging-related changes in the sensitivity of behavioral effects of the neonicotinoid pesticide clothianidin in male mice
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DOI：
10.1016/j.toxlet.2021.02.010
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发表时间：
2021-02-26
影响因子：
3.5
通讯作者：
Hoshi, Nobuhiko
中科院分区：
文献类型：
--
作者：
Hirano, Tetsushi;Miyata, Yuka;Hoshi, Nobuhiko
新烟碱类杀虫剂作为昆虫烟碱乙酰胆碱受体的激动剂，是全世界广泛使用的杀虫剂。尽管流行病学研究表明，老年人尿液中新烟碱类杀虫剂的检出量高于其他年龄组，但尚无关于新烟碱类杀虫剂对老年哺乳动物的风险的信息。本研究旨在调查新烟碱类杀虫剂噻虫胺 (CLO) 的行为影响与衰老相关的差异。我们对成年（12 周龄）和老年（90 周龄）小鼠急性施用低于 NOAEL 水平（5 毫克/千克）的 CLO，并进行了四项针对情绪行为的行为测试。此外，我们还测量了血液、大脑和尿液中 CLO 及其代谢物的浓度。所有行为测试中大多数参数都存在与年龄相关的变化，CLO显着降低了老年组旷场测试和高架十字迷宫测试中的运动活性，但在成年组中没有显着降低。与成人组相比，老年组中大多数 CLO 及其代谢物的浓度在血液和大脑中显着较高，而在尿液中略低。这些发现应有助于我们了解新烟碱类杀虫剂对哺乳动物的不利影响与年龄相关的差异。 (C) 2021 Elsevier B.V. 保留所有权利。
Neonicotinoids, which act as agonists of the nicotinic acetylcholine receptors of insects, are widely used pesticides worldwide. Although epidemiological studies revealed that the detection amounts of neonicotinoids in urine are higher in the elderly population than other age-groups, there is no available information regarding the risks of neonicotinoids to older mammals. This study was aimed to investigate aging-related differences in the behavioral effects of the neonicotinoid pesticide clothianidin (CLO). We acutely administered a sub-NOAEL level (5 mg/kg) of CLO to adult (12-week-old) and aging (90-week-old) mice and conducted four behavioral tests focusing on the emotional behavior. In addition, we measured the concentrations of CLO and its metabolites in blood, brain and urine. There were age-related changes in most parameters in all behavioral tests, and CLO significantly decreased the locomotor activity in the open field test and elevated plus-maze test in the aging group, but not in the adult group. The concentrations of most CLO and its metabolites were significantly higher in the blood and brain and were slightly lower in the urine in the aging group compared to the adult group. These findings should contribute to our understanding of age-related differences in the adverse effects of neonicotinoids in mammals. (C) 2021 Elsevier B.V. All rights reserved.