Measurement of Local Cerebral Blood Flow with [14C]Iodoantipyrine in the Mouse
Measurement of Local Cerebral Blood Flow with [14C]Iodoantipyrine in the Mouse
复制标题
[14C]碘安替比林测量小鼠局部脑血流量
DOI：
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发表时间：
1988
影响因子：
6.3
通讯作者：
L. Sokoloff
中科院分区：
文献类型：
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作者：
T. Jay;G. Lucignani;A. Crane;J. Jehle;L. Sokoloff
通过[14 C]碘安替比林方法测量小鼠的局部脑血流量。这种方法以前曾用于猴、狗、猫和大鼠，但将其应用于小鼠等小型哺乳动物需要特别注意潜在的误差来源。小鼠大脑体积小，需要特别注意快速取出和冷冻大脑，以尽量减少死后示踪剂在组织中扩散的影响。由于小动物动脉取样所需导管的直径/长度比相对较低，因此在测定动脉血中[14 C]碘安替比林浓度的时程时可能会出现大量误差，除非对导管中的滞后时间和死腔冲洗进行适当校正。在7只清醒小鼠中测量局部脑血流量，并适当注意尽量减少这些误差来源。发现这些值从胼胝体的48 ml/100 g/min到下丘的198 ml/100 g/min不等。结果表明，[14 C]碘安替比林法可用于测量小鼠局部脑血流量，该物种的值一般略高于大鼠。
Local cerebral blood flow was measured in the mouse by means of the [14C]iodoantipyrine method. This method has been previously used in the monkey, dog, cat, and rat, but its application to small mammals such as the mouse requires special attention to potential sources of error. The small size of the mouse brain requires special attention to the rapid removal and freezing of the brain to minimize effects of postmortem diffusion of tracer in the tissue. Because of the relatively low diameter/length ratios of the catheters needed for arterial sampling in small animals, substantial errors can occur in the determination of the time course of the [14C]iodoantipyrine concentration in the arterial blood unless corrections for lag time and dead space washout in the catheter are properly applied. Local cerebral blood flow was measured in seven awake mice with appropriate care to minimize these sources of error. The values were found to vary from 48 ml/100 g/min in the corpus callosum to 198 ml/100 g/min in the inferior colliculus. The results demonstrate that the [14C]iodoantipyrine method can be used to measure local cerebral blood flow in the mouse and that the values in that species are, in general, somewhat higher than those in the rat.