FACTORS INFLUENCING GROWTH + POLYSACCHARIDE FORMATION BY STAINS OF CHROMOBACTERIUM VIOLACEUM
FACTORS INFLUENCING GROWTH + POLYSACCHARIDE FORMATION BY STAINS OF CHROMOBACTERIUM VIOLACEUM
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DOI：
10.1128/jb.88.5.1433-1441.1964
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发表时间：
1964-01-01
影响因子：
3.2
通讯作者：
CORPE, WA
中科院分区：
文献类型：
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作者：
CORPE, WA
明胶角紫罗兰属菌株产生一种纤维状外多糖，形成嵌入生长细胞的基质。从凝胶状培养物中产生的稳定的非凝胶变体有两种类型，即失去产生多糖的能力的变体和失去将聚合物结合到基质中的能力的变体。在胰蛋白胨中生长的胶凝菌株不产生基质，而是将多糖游离到培养基中。胰蛋白胨的作用不是选择非明胶变体。当氨基酸取代氨作为氮源时，在限定培养基中的生长和多糖合成最大。当碳氮比为10：1时，菌体生长和多糖产量最佳。在以酪蛋白氨基酸为氮源生长的培养物中，所形成的聚合物发生解聚。钙离子刺激多糖形成。铁，虽然刺激生长，抑制多糖的形成。
Gelatinous C. violaceum strains elaborate a fibrous exopolysaccharide that forms a matrix in which growing cells are embedded. Stable nongelatinous variants arising from gelatinous cultures were of two types those that had lost the ability to produce polysaccharide and those that had lost the capacity to bind the polymer into a matrix. Gelatinous strains growing in tryptone did not produce a matrix, but rather elaborated polysaccharide free into the medium. The effect of tryptone was not one of selection of nongelatinous variants. Growth and polysaccharide synthesis in a defined medium were greatest when amino-acids were substituted for ammonia as a nitrogen source. Best growth and polysaccharide yield occurred when the ratio of carbohydrate to nitrogen was in the order of 10:1. Depoly-merization of formed polymer occurred in cultures grown with casamino acids as the nitrogen source. Calcium ion stimulated polysaccharide formation. Iron, although stimulating growth, inhibited polysaccharide formation.