Cold‐adapted bacteria for bioremediation of crude oil‐contaminated soil
Cold‐adapted bacteria for bioremediation of crude oil‐contaminated soil
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DOI：
10.1002/jctb.4814
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发表时间：
2016-08
影响因子：
3.4
通讯作者：
Jingxiu Wang;Junming Wang;Zhongzhi Zhang;Yongfeng Li;Beiyu Zhang;Zhiyong Zhang;Guang-qing Zhang
中科院分区：
文献类型：
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作者：
Jingxiu Wang;Junming Wang;Zhongzhi Zhang;Yongfeng Li;Beiyu Zhang;Zhiyong Zhang;Guang-qing Zhang
有许多寒冷气候的陆地场地受到石油烃污染。在这些地区，成功的生物修复在很大程度上依赖于在低温下具有生物降解能力的合适微生物。本研究旨在筛选低温适应细菌菌株并考察其降解石油污染物的能力。结果从寒冷环境的含油污泥中分离到11株冷适应菌。根据生长曲线和除油率，分别鉴定为金黄杆菌属、芽孢杆菌属和假单胞菌属的3株菌株对原油污染物具有较好的生物降解能力。三种细菌的最佳降解条件为10 °C，pH 7和盐度10 g L−1，原油浓度为1000 mg L− 1。在此条件下，8天后三种细菌的除油率分别为62.3%、61.6%和60.9%。在模拟生物修复试验中，使用含有5.8-10.6 g油kg−1土壤的人工污染土壤，在10 °C下生物修复150天后，实现了61-78%的油去除率。结论3株冷适应菌均能在低温下高效降解原油，适用于寒冷环境下石油污染土壤的生物修复。© 2015化学工业协会
BACKGROUND There are many cold climate terrestrial sites contaminated with petroleum hydrocarbons. Successful bioremediation in these regions relies heavily on suitable microorganisms with biodegrading capability at low temperatures. This work aims at screening cold-adapted bacteria strains and examining their capability of biodegrading petroleum contaminants. RESULTS Eleven strains of cold-adapted bacteria were isolated from oily sludge taken from a cold environment. Three strains, identified as Chryseobacterium, Bacillus and Pseudomonas, respectively, demonstrated high efficiency in biodegradation of crude oil pollutants based on the growth curves and oil removal rate. The optimal degradation conditions of the three bacteria were 10 °C, pH 7 and salinity of 10 g L−1 for a crude oil concentration at 1000 mg L−1. Under these conditions, the oil removal of the three bacteria after 8 days was 62.3, 61.6 and 60.9%, respectively. The strains were applied in simulated bioremediation tests using artificial contaminated soil containing 5.8–10.6 g oil kg−1 soil and achieved 61–78% oil removal after 150 days of bioremediation at 10 °C. CONCLUSION The three cold-adapted bacteria strains are capable of degrading crude oil efficiently at low temperatures and thus are suitable for bioremediation of petroleum contaminated soils in cold environments. © 2015 Society of Chemical Industry